Strain Information | |
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Image | |
BRC No. | RBRC06303 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.Cg-Malat1<tm1.1Shna>/ShnaRbrc |
Former Common name | Malat1 KO, B6-Malat1 KO, NEAT2(lacZ)(Acc.No.CDB0837K) |
H-2 Haplotype | |
ES Cell line | TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] |
Background strain | |
Appearance | |
Strain development | Developed by Shinichi Nakagawa, RIKEN Advanced Science Institute. TT2 ES cells were used to generate the knockout mice. A FRT-flanked neo cassette was removed by crossing with FLP mice. The mutant mice were backcrossed to C57BL/6N. |
Strain description | Malat1 ncRNA knock-out mice. Homozygous mutant mice are viable and fertile. |
Colony maintenance | Homozygote x Homozygote [or Crossing to C57BL/6NCrSlc] |
References | RNA, 18, 1487-1499 (2012). 22718948 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Malat1metastasis associated lung adenocarcinoma transcript 1 (non-coding RNA)19Malat1<tm1.1Shna>targeted mutation 1.1, Shinichi Nakagawa Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site19Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lacZbeta-galactosidase (E. coli)19 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lox71mutant lox sequence19lox71 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP19loxP |
Ordering Information | |
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Donor DNA | phage P1 lox71 site, E. coli lacZ, polyA, yeast FRT (flipase recombination target) site, phage P1 loxP site, polyA, mouse Malat1 genomic DNA |
Research application | Cell Biology Research Cre/loxP system FLP/frt system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | (1) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Acc.No.CDB0837K and Web. Site (https://large.riken.jp/distribution/mutant-list.html) is requested. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested (RNA. 2012 18(8):1487-99.). (3) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published. |
Depositor | RIKEN CDB (RIKEN CDB) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |