Strain Data Sheet

RBRC06312

Strain Information

Image
BRC No.RBRC06312
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameICR.Cg-Mesp2<tm7.1(mespb)Ysa>
Former Common nameZmpr2, Mesp2/mespb KI
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Yumiko Saga, National Institute of Genetics and Yukuto Yasuhiko, National Institute of Health Sciences. TT2 ES cells were used. ICR mixed background. Neo cassette was removed.
Strain descriptionMesp2-mespb knock-in mice. Exons 1 and 2 of Mesp2 were replaced with a zebrafish mespb ORF. Homozygous mutant mice die after birth.
Colony maintenanceHeterozygote x Wild-type [or Crossing to Jcl:MCH(ICR)]
ReferencesDevelopment, 129, 2473-2781 (2002). 11973278

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Mesp2mesoderm posterior 27Mesp2<tm7(mespb)Ysa>targeted mutation 1, Yumiko Saga

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Mespbmesoderm posterior 27Mespb

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP7loxP

Ordering Information

Donor DNAPhage P1 loxP, mouse Mesp2 genomic DNA, Zebrafish mespb cDNA
Research applicationCre/loxP system
Developmental Biology Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Development, 129, 2473-2781 (2002).The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
DepositorJun Kanno (National Institute of Health Sciences)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data