Strain Information | |
---|---|
Image | |
BRC No. | RBRC06317 |
Type | Targeted Mutation and Transgene |
Species | Mus musculus |
Strain name | C.Cg-Cables1<Tg(CAG-rtTA,CAG-tTS)138Utr> |
Former Common name | TAS, BALB-Cables1/TAS |
H-2 Haplotype | |
ES Cell line | |
Background strain | BALB/cAJcl |
Appearance | |
Strain development | Developed by Fumihiro Sugiyama, Laboratory animal resource center, University of Tsukuba in 2010. The transgene was coinjected into the pronuclei of B6D2F1 fertilized eggs. The transgenic mice were backcrossed to BALB/cAJcl for 14 times. |
Strain description | Insertional mutant mice of the Cables1 gene, arose from CAG-rtTA/CAG-tTS transgenic lines. Tg homozygous mice and about 50% of hemizygous mice show agenesis of the corpus callosum (ACC). |
Colony maintenance | Carrier x Noncarrier [BALB/cAJcl] |
References | Laboratory Investigation, 94, 321-330 (2014) . 24336072 Mammalian Genome, 21, 525-533 (2010) . 20967545 |
Health Report | |
---|---|
Examination Date / Room / Rack |
Gene | |
---|---|
Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Cables1CDK5 and Abl enzyme substrate 118Cables1<Tg(CAG-rtTA,CAG-tTS)138Utr>transgene insertion 1, University of Tsukuba Laboratory Animal Resource Center Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter rtTAE. coli Reverse tetracycline transactivator (Mutated tetrepressor DNA-binding domain and the transactivation domain of VP-16)18rtTAE. coli tet repressor (TetR), herpes simplex virus (HSV)VP16 activation domainCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter tTSE. coli Tetracycline repressor (tetR), human ZFP KRAB domain cDNA18tTSCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) |
Ordering Information | |
---|---|
Donor DNA | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), rtTA[E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain], tTS (E. coli Tetracycline repressor (tetR), human ZFP KRAB domain cDNA) |
Research application | Developmental Biology Research Tet system |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Truncated Cables1 Causes agenesis of the corpus callosum in mice. |
Depositor | Fumihiro Sugiyama (University of Tsukuba) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
---|
No Data |