Strain Data Sheet
ANIMAL SEARCH SYSTEM
Data update: Feb 22, 2017
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RBRC No. RBRC06344  
Type Targeted MutationCartagena  
Species No Data  
Strain name C57BL/6-App<tm3(NL-G-F)Tcs>  
Former Common name APP, B6-App/NL-G-F KI  
H-2 Haplotype No Data  
ES cell line iTL IC1 [C57BL/6NTac]  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Takashi Saito and Takaomi C. Saido, RIKEN Brain Science Institute in 2009. C57BL/6 derived ES cells were used to generate the mutant mice. A floxed neo cassette was removed by crossing with EIIa-cre mice (B6.FVB-Tg(EIIa-cre)C5379Lmgd/J). C57BL/6 background.  
Strain description App knock-in mice having Swedish mutation (NL) (RBRC06342), Swedish/Iberian (NL-F) mutations (RBRC06343) and Swedish/Iberian/Arctic mutations (NL-G-F) (RBRC06344). Homozygous mutant mice are viable and fertile.  
Colony maintenance Heterozygote x Heterozygote, Wild-type [or Crossing to C57BL/6J(Crlj)]  
Health Report
Gene Details
Promoter No Data  
 1 Symbol App  
Symbol name amyloid beta (A4) precursor protein  
Chromosome 16  
Common name No Data  
Symbol description No Data  
 2 Symbol FRT  
Symbol name yeast FRT (flippase recombination target) site  
Chromosome 16  
Common name No Data  
Symbol description No Data  
 3 Symbol loxP  
Symbol name phage P1 loxP  
Chromosome 16  
Common name No Data  
Symbol description No Data  
References   
Research applications Cre/loxP system,
FLP/frt system,
Mouse Models for Human Disease  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the specific Approval Form.
RIKEN BRC does not provide any for-profit organization with the BIOLOGICAL RESOURCE. For-profit organizations must contact the DEPOSITOR (saido@brain.riken.jp) for a licensing contract. RECIPIENT shall supply DEPOSITOR with a written report about all research results related to the BIOLOGICAL RESOURCE as outlined in the Research Plan. RECIPIENT will have the right to publish and disclose the results of the research and will submit the intended publication to DEPOSITOR at least thirty (30) days prior to submission of the abstracts and manuscripts. DEPOSITOR may within this 30-day period request RECIPIENT, in writing, to delete any reference to DEPOSITOR's CONFIDENTIAL information. If any invention is conceived and reduced to practice by RECIPIENT in the performance of the Research Plan involving the BIOLOGICAL RESOURCE during the term of this MTA, RECIPIENT agrees to promptly inform the invention to DEPOSITOR and the parties will consult each other to determine inventorship and ownership of the invention based on the respective parties contribution to the invention, before filing an application for a patent. In the case of a jointly owned application, the parties will separately enter into a joint application agreement including the sharing of ownership, patent costs and licensing income, as well as the responsible party for the application procedures etc. RECIPIENT agrees to acknowledge Takashi SAITO and Takaomi C. SAIDO, according to generally accepted authorship attributions, as co-authors in the first domestic oral presentation, first international oral presentation, and first written international publication as related to the BIOLOGICAL RESOURCE unless otherwise requested by the DEPOSITOR. Thereafter, RECIPIENT agrees to acknowledge Takashi SAITO and Takaomi C. SAIDO in an acknowledgement section, and does not need to co-author Takashi SAITO and Takaomi C. SAIDO. RECIPIPENT also agrees to cite Nature Neuroscience 17 and 661-663 (2014)in all publications obtained using BIOLOGICAL RESOURCE. RECIPIENT agrees to acknowledge DEPOSITOR in all oral presentations and written publications as related to the BIOLOGICAL RESOURCE. RECIPIENT shall keep confidential any or all information marked CONFIDENTIAL that is received from DEPOSITOR and related to the BIOLOGICAL RESOURCE including oral discussions if they are reduced to writing within thirty (30) days and are marked "CONFIDENTIAL". The BIOLOGICAL RESOURCE is the property of the RIKEN(The institute of physical and chemical research).  
Additional information
 1 MTA(English), MTA(Japanese), APPROVAL FORM(English), APPROVAL FORM(Japanese)
Lab HP (Japanese)  
 2 Mouse of the Month Sep 2014  
 3 Genotyping protocol <PCR>  
Depositor Saido, C. Takaomi (The Institute of Physical and Chemical Research)  Saido, C. Takaomi 
Strain Status /
Availability
(Expected delivery)

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