Strain Data Sheet
ANIMAL SEARCH SYSTEM
Data update: Sep 20, 2018
Health Report   PCR Protocol   Thawing Method
embryos / sperm >
  Mouse Marking
Figure
  Order
RBRC No. RBRC06352  
Type Gene Trap  
Species Mus musculus  
Strain name B6.129S4-Asf1a<Gt(S7-4B1)Sor>/Skmz  
Former Common name B6-CIA-I, B6-Asf1a Gt  
H-2 Haplotype No Data  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Kazuhisa Sekimizu, Graduate School of Pharmaceutical Sciences in 2007. CIA-II (RBRC06350): Originated from Asf1b<Gt(ROSABetageo)437Muv> gene trapped mice (EMMA ID:01154). Backcrossed to C57BL/6. CIA-I (RBRC06351 and RBRC06352): Generated by using Asf1a<Gt(S7-4B1)Sor> gene trapped ES cells (MMRRC #13934-USD/S7-4B1). The mutant mice were backcrossed to ICR and C57BL/6 mice, respectively.  
Strain description CIA-II(Asf1b) gene trapped mice (B6 background)(RBRC06350), CIA-I (Asf1a) gene trapped mice (ICR mixed background: RBRC06351 and B6 background: RBRC06352). Homozygous and heterozygous mutant mice for CIA-II gene show no obvious abnormality. Homozygous mutant mice for CIA-I of ICR mixed and C57BL/6 background were perinatal and embryonic (around E12.5) lethal, respectively.  
Colony maintenance Heterozygote x Wild-type [C57BL/6JJcl]  
Health Report
Gene Details
Promoter No Data  
 1 Symbol Asf1a  
Symbol name ASF1 anti-silencing function 1 homolog A (S. cerevisiae)  
Chromosome 10  
Common name No Data  
Symbol description No Data  
 2 Symbol Frt  
Symbol name yeast FRT (flippase recombination target) site  
Chromosome 10  
Common name No Data  
Symbol description No Data  
 3 Symbol GH  
Symbol name Growth hormone polyA (Bovine)  
Chromosome 10  
Common name No Data  
Symbol description No Data  
 4 Symbol LTR  
Symbol name retrovirus LTR (viral long terminal repeat)  
Chromosome 10  
Common name No Data  
Symbol description No Data  
 5 Symbol SA  
Symbol name Splice acceptor (adenovirus)  
Chromosome 10  
Common name No Data  
Symbol description No Data  
 6 Symbol SD  
Symbol name adenovirus splice donor (SD)  
Chromosome 10  
Common name No Data  
Symbol description No Data  
 7 Symbol lacZ  
Symbol name beta-galactosidase (E. coli)  
Chromosome 10  
Common name No Data  
Symbol description No Data  
 8 Symbol neo  
Symbol name neomycin resistance gene (E. coli)  
Chromosome 10  
Common name No Data  
Symbol description No Data  
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)  
 9 Symbol Hyg  
Symbol name hygromycin phasphotransferase (E. coli)  
Chromosome 10  
Common name No Data  
Symbol description No Data  
References Genes Dev. 1991 Sep;5(9):1513-23.

  
Promoter traps in embryonic stem cells: a genetic screen to identify and mutate developmental genes in mice.
Promoter traps in embryonic stem cells: a genetic screen to identify and mutate developmental genes in mice.
Promoter traps in embryonic stem cells: a genetic screen to identify and mutate developmental genes in mice.
Research applications Developmental Biology Research,
FLP/frt system,
Fluorescent Proteins/lacZ System  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
後日指定。 (will be announced).
The use of the BIOLOGICAL RESOURCE is strictly limited to academic, non-profit research institutes for academic non-profit research purposes. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, a mention of the Sanger Institute Gene Trap Resource as the source of the original allele are requested.  
Additional information
 1 Lab HP (Japanese)  
 2 Genotyping protocol <PCR>  
Depositor Sekimizu, Kazuhisa (The University of Tokyo)  Sekimizu, Kazuhisa 
Strain Status /
Availability
(Expected delivery)

back Back
RIKEN BioResource Research Center

English page click here ANIMAL SEARCH SYSTEM