Strain Information | |
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Image | |
BRC No. | RBRC06358 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.129P2-Scn1a<tm1.1Kzy> |
Former Common name | Scn1a KI, B6-Scn1a KI |
H-2 Haplotype | |
ES Cell line | E14 [129P2/OlaHsd] |
Background strain | |
Appearance | |
Strain development | Developed by Ikuo Ogiwara and Kazuhiro Yamakawa, RIKEN Brain Science Institute in 2007. E14 ES cells were used to generate the mutant mice. After ackcrossing to C57BL/6J (JAX) for 5 times, a frt flanked sequence were removed by crossing with CAG-FLPe mice (B6 background), and then the mice were backcrossed to C57BL/6J(JAX) over 9 times. |
Strain description | Scn1a GENE R1407* nonsense mutation knock-in mice. Homozygous mutant mice show ataxia from around 10 days after birth and die up before 25 days-old due to malnutrition. Heterozygous mutant mice show epileptic seizure from around 14 days after birth, and about 30-50% mice die a sudden death at 20 to 40 days-old. Model mice for dravet syndrome. |
Colony maintenance | Heterozygote x Wild-type [C57BL/6J(Crlj)] |
References | Neurobiol. Dis., 16, 29-40 (2013). 22986304 Epilepsia., 53, 1140-1145 (2012). 22578034 J. Neurosci., 27, 5903-5914 (2007). 17537961 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Scn1asodium channel, voltage-gated, type I, alpha2Scn1a<tm1.1Kzy>targeted mutation 1.1. Kazuhiro Yamakawa Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site2Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP2loxP |
Ordering Information | |
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Donor DNA | yeast FRT (flipase recombination target) site, bacteriophage P1 loxP site, mouse Scn1a mutant (R1407*) gene |
Research application | Cre/loxP system FLP/frt system Neurobiology Research |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurosci., 27, 5903-5914 (2007).The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
Depositor | Kazuhiro Yamakawa (RIKEN) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Mouse of the Month Oct 2014 |
BRC mice in Publications |
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No Data |