Strain Information | |
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Image | |
BRC No. | RBRC06398 |
Type | Targeted Mutation![]() |
Species | Mus musculus |
Strain name | B6;129P2-Rnf123<tm1Kei> |
Former Common name | Kpc1 KO, B6-Kpc1 KO, B6;129P2-Kpc1<tm1Kei> |
H-2 Haplotype | |
ES Cell line | E14 [129P2/OlaHsd] |
Background strain | |
Appearance | |
Strain development | Developed by Keiichi Nakayama, Medical Institute of Bioregulation, Kyushu University. E14 ES cells were used. The mutant mice were crossed to C57BL/6J. |
Strain description | Kpc1 (Rnf123) gene knockout mice. Exons 34 to 37 containing a RING finger domain were replaced with a PGK-neo (loxP-neo) cassette. |
Colony maintenance | Homozygote x Homozygote [or Crossing to C57BL/6JJcl] |
References | NATURE CELL BIOLOGY, 6, 1229-1235 (2004). 15531880 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP9loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP9loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)9mouse phosphoglycerate kinase promoter (PGK promoter) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 polyA signal9 |
Ordering Information | |
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Donor DNA | phage P1 loxP sites, mouse phosphoglycerate kinase promoter, E. coli Neomycin resistance gene, SV40 polyA signal, mouse Rnf123 genomic DNA |
Research application | Cancer Research Cell Biology Research Cre/loxP system |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. |
Depositor | Keiichi Nakayama (Kyushu University) |
Strain Status | ![]() ![]() |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |