Strain Data Sheet

RBRC06410

Strain Information

Image
BRC No.RBRC06410
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Nfkbiz<tm1.1Muta>
Former Common nameIκB-ζ flox/flox, B6-Nfkbiz floxed
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strain
Appearance
Strain developmentDeveloped by Tatsushi Muta, Graduate School of Life Sciences, Tohoku University. Bruce 4 ES cells were used to generate the mutant mice. A frt-neo cassette was removed by crossing with CAG-FLPe (B6) mice.
Strain descriptionNfkbiz gene floxed mice. Exons 5 to 7 were flanked by loxP sites.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJmsSlc]
ReferencesImmunity, 38, 450-460 (2013). 23453632

Health Report

Examination Date / Room / Rack2024/01/09Room:3-BRack:L
2023/10/10Room:3-BRack:L
2023/07/10Room:3-BRack:L
2023/04/10Room:3-BRack:L
2023/03/24Room:3-BRack:L
2023/01/10Room:3-BRack:L
2022/10/11Room:3-BRack:L
2022/07/11Room:3-BRack:L
2022/04/11Room:3-BRack:L

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Nfkbiznuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, zeta16Nfkbiz<tm1.1Muta>targeted mutation 1.1, Tatsushi Muta

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site16Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP16loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP16loxP

Ordering Information

Donor DNAP1 phage loxP sites, yeast FRT (flippase recombination target) site, mouse Nfkbiz genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
DepositorTakashi Maruyama (Gifu University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (3 to 6 months)
Additional Info.Mouse of the Month Apr 2017
Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Taylor TC, Coleman BM, Arunkumar SP, Dey I, Dillon JT, Ponde NO, Poholek AC, Schwartz DM, McGeachy MJ, Conti HR, Gaffen SL.
IκBζ is an essential mediator of immunity to oropharyngeal candidiasis.
Cell Host Microbe (2023) 37725983

Kakiuchi N, Yoshida K, Uchino M, Kihara T, Akaki K, Inoue Y, Kawada K, Nagayama S, Yokoyama A, Yamamoto S, Matsuura M, Horimatsu T, Hirano T, Goto N, Takeuchi Y, Ochi Y, Shiozawa Y, Kogure Y, Watatani Y, Fujii Y, Kim SK, Kon A, Kataoka K, Yoshizato T, Nakagawa MM, Yoda A, Nanya Y, Makishima H, Shiraishi Y, Chiba K, Tanaka H, Sanada M, Sugihara E, Sato TA, Maruyama T, Miyoshi H, Taketo MM, Oishi J, Inagaki R, Ueda Y, Okamoto S, Okajima H, Sakai Y, Sakurai T, Haga H, Hirota S, Ikeuchi H, Nakase H, Marusawa H, Chiba T, Takeuchi O, Miyano S, Seno H, Ogawa S.
Frequent mutations that converge on the NFKBIZ pathway in ulcerative colitis.
Nature 577(7789) 260-265(2020) 31853061

Terui H, Yamasaki K, Wada-Irimada M, Onodera-Amagai M, Hatchome N, Mizuashi M, Yamashita R, Kawabe T, Ishii N, Abe T, Asano Y, Aiba S.
Staphylococcus aureus skin colonization promotes SLE-like autoimmune inflammation via neutrophil activation and the IL-23/IL-17 axis.
Sci Immunol 7(76) eabm9811(2022) 36306369

Murakami T, Takahata Y, Hata K, Ebina K, Hirose K, Ruengsinpinya L, Nakaminami Y, Etani Y, Kobayashi S, Maruyama T, Nakano H, Kaneko T, Toyosawa S, Asahara H, Nishimura R.
Semaphorin 4D induces articular cartilage destruction and inflammation in joints by transcriptionally reprogramming chondrocytes.
Sci Signal 15(758) eabl5304(2022) 36318619