Strain Data Sheet

RBRC06457

Strain Information

Image
BRC No.RBRC06457
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Pax6<tm1(iCre)Fmur>
Former Common namePax6iCre, B6-Pax9/cre KI
H-2 Haplotype
ES Cell lineRENKA [C57BL/6NCrlCrlj]
Background strainC57BL/6NCrl
Appearance
Strain developmentDeveloped by Fujio Murakami, Graduate School of Frontier Biosciences, Osaka Univerisyt in 2012. RENKA ES cells were used to generate the mutant mice. A frt-flanked PGK-neo cassette was removed by crossing with ACTB-FLPe mice (C57BL/6NCrl background).
Strain descriptionPax6 hgene Cre knock-in mice. A codon improved Cre-polyA was inserted into the start methionine site.
Colony maintenanceHeterozygote x Wild-type [C57BL/6NCrlCrlj]
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Pax6paired box 62Pax6<tm1(iCre)Fmur>targeted mutation 1, Fujio Murakami

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site2Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
iCrephage P1 codon improved Cre (iCre)2iCre

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 PolyA signal2

Ordering Information

Donor DNABacteriophage P1 codon improved Cre (iCre), SV40 PolyA signal, Saccharomyces cerevisiae Flp recombinase target (FRT) site, Mouse Paired box 6 (PAX6) genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced.) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR (Dr. Fujio Murakami, Osaka University) and DEVELOPER (Dr. Kenji Sakimura, Nigata University) is requested. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
DepositorFujio Murakami (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data