Strain Data Sheet
RBRC06467
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC06467 |
| Type | Transgene |
| Species | Mus musculus |
| Strain name | C57BL/6-Tg(Prnp-Sirt1,Cryaa-EGFP)1Ksh |
| Former Common name | PrP-Sirt1 Tg, B6-Prnp-Sirt1/Cryaa-GFP Tg |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | Developed by Makoto Kinoshita, Nagoya University Graduate School of Science (2008-9). Prnp-Sirt1 and alpha A-crystallin EGFP transcription units were co-injected into the C57BL/6J mouse oocytes. |
| Strain description | PrP-Sirt1 transgenic mice having a mouse Sirt1 gene under the control of mouse prion gene promoter (MoPrP). The MoPrP-Sirt1 transcription unit was co-injected with a mouse alpha A-crystallin promoter-driven EGFP cDNA. |
| Colony maintenance | Carrier x Nocarrier [C57BL/6JJmsSlc] |
| References | Mol. Brain, 7(1):62 (2014). 25167838 F1000Res. 4:130 (2015). 26167274 Stroke 45, 3403-3411 (2014). 25213338 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)UNEGFPmouse Cryaa promoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Sirt1sirtuin 1UNSirt1mouse Prnp promoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 polyA signalUN |
Ordering Information | |
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| Donor DNA | Mouse Prnp gene promoter, Mouse Sirt1 gene cDNA, Mouse aA-crystallin gene promoter, Aequorea victoria (humanized) enhanced green fluorescent protein (GFP) gene cDNA, SV40 PolyA signal |
| Research application | Fluorescent Proteins/lacZ System Neurobiology Research |
| Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Brain, 7(1):62 (2014).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. For profit use of the BIOLOGICAL RESSOURCE, the RECIPIENT must contact with the Intellectual Property Office, Nagoya University to obtain a prior written consent. |
| Depositor | Makoto Kinoshita (Nagoya University) |
| Strain Status |  Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Mouse of the Month Feb 2015 |
BRC mice in Publications |
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| No Data |