Strain Information | |
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Image | |
BRC No. | RBRC06506 |
Type | Transgene |
Species | Mus musculus |
Strain name | ICR.Cg-Tg(CAG-cat,-3xFLAG/Dazl)4 |
Former Common name | CAG-CAT-3FLAG・Dazl#4, ICR-CAG-FLAG/Dazl Tg#4 |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Developed by Atsushi Suzuki, Faculty of Engineering, Yokohama National University in 2012. The transgene was injected into the pronuclei of B6C3F1 fertilized eggs. ICR mixed background. |
Strain description | 3xFLAG-DAZL conditional transgenic mice. Line 3 (RBRC06505)(main line), Line 4 (RBRC06506). |
Colony maintenance | Carrier x Noncarrier [or Crossing to Jcl:MCH(ICR)] |
References | Proc. Natl. Acad. Sci. USA, 92, 160-164 (1995). 7816809 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Dazldeleted in azoospermia-likeUNDazlCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter FLAGFLAG tag (synthetic)UNFLAG Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter catE. coli chloramphenicol acetyltransferase (CAT)UN Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxPUNloxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxPUNloxP |
Ordering Information | |
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Donor DNA | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), phage P1 loxP sites, E. coli chloramphenicol acetyltransferase, 3xFLAG tag sequence, Mouse Daz-like gene cDNA, polyA |
Research application | Cre/loxP system |
Specific Term and Conditions | In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. |
Depositor | Atsushi Suzuki (Yokohama National University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |