Strain Data Sheet
ANIMAL SEARCH SYSTEM
Data update: Dec 25, 2017
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RBRC No. RBRC06510  
Type TransgeneCartagena  
Species No Data  
Strain name C57BL/6J-Tg(tetO-G-CaMP7,-DsRed2)572Bsi  
Former Common name TRE-G-CaMP7, B6-tetO-GCMP7/DsRed Tg#572  
H-2 Haplotype No Data  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Yasunori Hayashi, RIKEN Brain Institute. The transgene was injected into the pronuclei of C57BL/6J fertilized eggs.  
Strain description TRE-C-CaMP7/DsRed2 transgenic mice. These transgenic mice express G-CAMP7 and DsRed2 under the control of the TRE (tetracycline response element) promoter.  
Colony maintenance Carrier x Noncarrier [or Crossing to C57BL/6JJmsSlc]  
Health Report No Data  
Gene Details
Promoter No Data  
 1 Symbol No Data  
Symbol name SV40 polyA signal  
Chromosome UN  
Common name No Data  
Symbol description No Data  
 2 Symbol DsRed2  
Symbol name Red Fluorescent Protein (Discosoma sp.)  
Chromosome UN  
Common name No Data  
Symbol description No Data  
 3 Symbol T2A  
Symbol name Thosea asigna virus 2A peptide (insect)  
Chromosome UN  
Common name No Data  
Symbol description No Data  
Promoter E. coli TRE promoter (tetracycline responsive promoter)  
 4 Symbol GCaMP7  
Symbol name RSET tag, chicken myosin light chain kinase (M13) cDNA, Jellyfish circularly permutated EGFP (cpEGFP) cDNA, rat calmodulin (CaM) cDNA  
Chromosome UN  
Common name No Data  
Symbol description No Data  
References   
Research applications Fluorescent Proteins/lacZ System,
Tet system  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
PLoS One, 10(5): e0125354, 2015. Sato et al. Generation and imaging of transgenic mice that express G-CaMP7 under a tetracycline response element.
For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.  
Additional information
 1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.  
 2 Genotyping protocol <PCR>  
Depositor Yasunori, Hayashi (RIKEN Brain Science Institute)  Yasunori, Hayashi 
Strain Status /
Availability
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