Strain Information | |
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Image | |
BRC No. | RBRC06519 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | C57BL/6N-Crh<tm1(venus)Ksak> |
Former Common name | CRF-Venus, B6-Crh/venus KI |
H-2 Haplotype | |
ES Cell line | RENKA [C57BL/6NCrlCrlj] |
Background strain | C57BL/6NJcl |
Appearance | |
Strain development | Developed by Keiichi Itoi, Graduate School of Information Sciences, Tohoku University and Kenji Sakimura, Brain Research Institute, Niigata University in 2011. RENKA ES cells were used to generate the mutant mice. |
Strain description | Crh (CRF, Corticotropin releasing factor) gene venus knock-in mice. A Venus-frt-flanked PGK-neo cassette was inserted at the translation initiation site of the CRF gene. |
Colony maintenance | Heterozygote x Wild-type [C57BL/6NJcl] |
References | Endocrinology. 155. 4054-4060 (2014). 25057791 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site3Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site3Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter VenusYellow Fluorescent Protein (Aequorea victoria)3Venus Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)3mouse Phosphoglycerate kinase promoter (PGK promoter), phage T7 gb2 promoter |
Ordering Information | |
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Donor DNA | A. coerulescens Venus mutant GFP cDNA, yeast FRT (flipase recombination target) sites, mouse phosphoglycerate kinase promoter (PGK promoter), phage T7 gb2 promoter(synthetic), E. coli Neomycin resistance gene, mouse Crh genomic DNA |
Research application | FLP/frt system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Endocrinology. 155. 4054-4060 (2014).In publishing the research results obtained by use of the BIOLOGICAL RESOURCE containing Venus, an acknowledgment to Dr. Atsushi Miyawaki and a citation of the following literature(s) designated by the DEPOSITOR are requested. Nat. Biotechnol., 20, 87-90 (2002). |
Depositor | Keiichi Itoi (Tohoku University) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |