Strain Data Sheet

RBRC06523

Strain Information

Image
BRC No.RBRC06523
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Cthrc1<tm1.2Suna>
Former Common nameCthrc1 sKO, B6-Cthrc1 KO
H-2 Haplotype
ES Cell lineiTL BA1[(C57BL/6N x 129S6/SvEvTac)F1]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Sunao Takeshita, National Center for Geriatrics and Gerontology. (C57BL/6NTac x 129S6/SvEvTac)F1 derived ES cells were used to generate the mutant mice. C57BL/6 background. Floxed mice (RBRC06522): A frt-flanked neo cassette was removed by crossing with CAG-FLPe mice. KO mice (RBRC06523): A frt-flanked neo cassette was removed by crossing with CAG-FLPe mice, and then the exon 2 was removed by crossing with CAG-Cre mice.
Strain descriptionCthrc1 gene floxed mice (RBRC06522)(Exon 2 was flanked by loxP sites). Cthrc1 gene KO mice (RBRC06523)(Exon 2 was deleted).
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]
ReferencesJ Clin Invest., 123, 3914-3924 (2013). 23908115

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Cthrc1collagen triple helix repeat containing 115Cthrc1<tm1.2Suna>targeted mutation 1.2, Sunao Takeshita

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site15Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP15loxP

Ordering Information

Donor DNAMouse Cthrc1 gene, yeast FRT (flipase recombination target) site, P1 phage loxP site
Research applicationCancer Research
Cre/loxP system
FLP/frt system
Hematological Research
Metabolism Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Clin. Invest., 123, 3914-3924 (2013).
DepositorKyoji Ikeda (National Center for Geriatrics and Gerontology)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data