Strain Information | |
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Image | |
BRC No. | RBRC06560 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6J.129P2-Brsk2<tm1Mkt>/28Yhi |
Former Common name | SAD-A delta ex2 28J KO, B6J-Brsk2 KO#28 |
H-2 Haplotype | |
ES Cell line | E14 [129P2/OlaHsd] |
Background strain | C57BL/6JJmsSlc |
Appearance | |
Strain development | Developed by Hiroyuki Niida and Makoto Nakanishi, Nagoya City University Medical School in 2005. Maintained by Institute for Developmental Research, Aichi Prefectural Colony in 2006. E14 ES cells were used to generate the mutant mice. |
Strain description | SAF-A (Brsk2) gene knockout mice. Exon 2 was replaced with a loxP-Pgk-neo-loxP cassette. Homozygous mutant mice die within 0-1 day after birth. Line 52J (RBRC06559), 28J (RBRC06560), 52N(RBRC06561), 28N(RBRC06562). Heterozygous females of C57BL/6J background cannot nurse pups. |
Colony maintenance | Heterozygote (female) x Wild-type (male)[C57BL/6JJmsSlc] |
References |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP7loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP7loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)7mouse phosphoglycerate kinase promoter (PGK promoter) |
Ordering Information | |
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Donor DNA | phage P1 loxP sites, mouse Phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse Brsk2 genomic DNA |
Research application | Cre/loxP system Developmental Biology Research Neurobiology Research |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. |
Depositor | Keiko Nakanishi (Aichi Prefectural Colony) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |