Strain Data Sheet

RBRC09650

Strain Information

Image
BRC No.RBRC09650
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(Slc1a2-G-CaMP7)Bsi
Former Common nameGLT1-G-CaMP7, G7NG817
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Hajime Hirase, Brain Science Institute, RIKEN in 2016. A BAC DNA construct was injected to fertilized eggs derived from C57BL/6J. Mice were maintained in this genetic background. Homozygous mice are viable and fertile.
Strain descriptionG-CaMP7 transgenic mice under the control of Slc1a2 (GLT-1) promoter. Astrocytes and a subset of cortical neurons express G-CaMP7.
Colony maintenance
ReferencesPLoS One, 7, e51286, (2012). 23240011
Nat Commun. (2016) 7:11100. DOI: 10.1038/ncomms11100 27000523
J. Neurosci., 20;27, 6607-6619 (2007). 17581948
Sci. Rep., 11, 8150 (2021). 33854148
Proc. Natl. Acad. Sci. USA, 116(22):11010-11019 (2019). 31097598

Health Report

Examination Date / Room / Rack2024/01/09Room:3-BRack:F
2023/10/10Room:3-BRack:F
2023/07/10Room:3-BRack:F
2023/04/10Room:3-BRack:F

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) siteUNFrt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GCaMP7RSET tag, chicken myosin light chain kinase (M13) cDNA, Jellyfish circularly permutated EGFP (cpEGFP) cDNA, rat calmodulin (CaM) cDNAUNGCaMP7mouse Slc1a2 promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HBB2rabbit beta-globin polyAUNHBB2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
WPREWoodchuck hepatitis virus posy-transcriptional regulatory element (WPRE)UNWPRE

Ordering Information

Donor DNAmouse Slc1a2 genomic DNA, GCaMP7 (RSET tag, chicken myosin light chain kinase (M13) cDNA, Jellyfish circularly permutated EGFP (cpEGFP) cDNA, rat calmodulin (CaM) cDNA), woodchuck hepatitis virus WPRE (woodchuck hepatitis virus posttranscriptional regulatory element) sequence, rabbit Beta globin polyA, yeast FRT (flippase recombination target) site, Phage P1 loxP site derived from pBACe3.6 vector, BAC clone RP23-361H22
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the above literature designated by the DEPOSITOR is recommended. Nat Commun. (2016) 7:11100. DOI: 10.1038/ncomms11100
DepositorHajime Hirase (RIKEN)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (3 to 6 months)
Additional Info.Mouse of the Month Aug 2017
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Holstein-Rønsbo S, Gan Y, Giannetto MJ, Rasmussen MK, Sigurdsson B, Beinlich FRM, Rose L, Untiet V, Hablitz LM, Kelley DH, Nedergaard M.
Glymphatic influx and clearance are accelerated by neurovascular coupling.
Nat Neurosci (2023) 37264158

Monai H, Wang X, Yahagi K, Lou N, Mestre H, Xu Q, Abe Y, Yasui M, Iwai Y, Nedergaard M, Hirase H.
Adrenergic receptor antagonism induces neuroprotection and facilitates recovery from acute ischemic stroke.
Proc Natl Acad Sci U S A 116(22) 11010-11019(2019) 31097598