Strain Information | |
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Image | |
BRC No. | RBRC09802 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6;129(FVB)-Sptlc2<tm1.1Yhir> |
Former Common name | |
H-2 Haplotype | |
ES Cell line | R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>] |
Background strain | C57BL/6JJcl |
Appearance | |
Strain development | Developed by Yoshio Hirabayashi, RIKEN Brain Science Institute in collaboration with Osaka University. R1 ES cell line [(129X1/SvJ x 129S1/Sv)F1] was used. Exon 3 was replaced with a fragment loxP-neo-loxP-Exon3-loxP. Neo fragment was removed by crossing with an EIIa-Cre mouse strain. |
Strain description | Sptlc2(serine palmitoyltransferase, long chain base subunit 2)floxed mice. Homozygous null mutants are embryonically lethal. |
Colony maintenance | |
References | Cell Metab., 24(6):820-834 (2016). 27818258 Exp. Anim., 58, 515-524 (2009). 19897935 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Sptlc2serine palmitoyltransferase, long chain base subunit 212Sptlc2<tm1Yhir>targeted mutation 1, Yoshio Hirabayashi Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP12loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP12loxP |
Ordering Information | |
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Donor DNA | Phage P1 loxP sites, mouse Sptlc2 genomic DNA |
Research application | |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Exp. Anim., 58, 515-524 (2009).The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. |
Depositor | Yoshio Hirabayashi (RIKEN) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Mouse of the Month Jun 2017 Genotyping protocol -PCR- Necessary documents for ordering: |
BRC mice in Publications |
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No Data |