Strain Data Sheet

RBRC10172

Strain Information

Image
BRC No.RBRC10172
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6;Cg-Sftpc<tm1(cre/ERT2)Yasu>
Former Common nameSftpC-CreERT2
H-2 Haplotype
ES Cell lineV6.5 [(C57BL/6J x 129S4/SvJae)F1]
Background strain
Appearance
Strain developmentDeveloped by Hirofumi Shibata and his colleagues in 2015, and deposited by Yasuhiro Yamada, Center for iPS Cell Research and Application, Kyoto University. V6.5 ES cells derived from (C57BL/6 x 129S4/SvJae)F1 were used. Mixed genetic background.
Strain descriptionKnock-in mouse of CreERT2 at the translation initiation codon of the SftpC gene. Cre/loxP recombination can be induced by the administration of Tamoxifen.
Colony maintenance
References

Health Report

Examination Date / Room / Rack2024/02/19Room:4-ARack:F
2023/11/20Room:4-ARack:F
2023/08/28Room:4-ARack:F
2023/08/21Room:4-ARack:F
2023/06/19Room:4-ARack:F
2023/05/22Room:4-ARack:F
2023/02/20Room:4-ARack:F
2022/11/21Room:4-ARack:F
2022/09/05Room:4-ARack:F
2022/08/22Room:4-ARack:F
2022/05/23Room:4-ARack:F

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Sftpcsurfactant associated protein C14Sftpc<tm1(cre/ERT2)Yasu>targeted mutation 1, Yasuhiro Yamada

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
BsdAspergillus terreus blasticidin resistance gene (Bsd)14Bsd

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
ERT2estrogen receptor (ER), human ER with the triple mutation G400V/M543A/L544A)14ERT2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HBB2rabbit beta-globin polyA14HBB2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
crePhage P1 Cre recombinase14cre

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 PolyA signal14

Ordering Information

Donor DNAP1 Bacteriophage Cre recomibinase, human Estrogen receptor binding domain (ERT2)cDNA, Rabbit globin pA(CreERT2), D6 Bacteriophage rox site, mouse PGK promoter, Aspergillus terreus Blasticidin S deaminase gene (Bsd), Simian virus (SV40) pA, mouse Sftpc genomic DNA
Research applicationCre/loxP system
Specific Term and ConditionsThe RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEVELOPER. The BIOLOGICAL RESOURCE shall be used only for academic research purposes by a non-profit organization.
DepositorYasuhiro Yamada (Kyoto University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (3 to 6 months)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data