Strain Data Sheet
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Data update: Nov 22, 2017
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RBRC No. RBRC00169  
Type Targeted Mutation CongenicCartagena   12wks,♂
Species Mus musculus  
Strain name B6.Cg-Tnc<tm1Sia>/Rbrc  
Former Common name C57BL/6N-TgH(Tnc), B6-TN, TNKO  
H-2 Haplotype No Data  
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]  
Background strain C57BL/6N Jcl obtained from Japan Clea.  
Appearance
 1 Appearance black  
Genotype a/a, B/B, C/C  
Strain development Congenic strains were developed by Dr. M. Kusakabe, Cell Biology Laboratory, Life Science Tsukuba Research Center, RIKEN since 1992. A lacZ gene and neomycin resistant cassette was transfered into TT2 ES cells to replace the exon 2, just before the translation initiation codon of the Tnc gene. This strain was backcrossed to C57BL/6N.  
Strain description Tenascin C knock-out mice. This strain is useful for studying the biological function of extracellular matrix tenascin C in vivo, especially in the field of tumor development, tumor metastasis, wound healing, tissue regeneration, angiogenesis, tissue interaction etc. The tenascin C deficient congenic strains were developed by successive backcrossing with A/J (RBRC00007), BALB/cA (RBRC00067), C57BL/6N (RBRC00169), C3H/HeN (RBRC00110) and GRS/A (RBRC00193).  
Colony maintenance Homozygote x Homozygote
Homozygous mutant mice are viable and fertile.  
Health Report
Gene Details
Promoter No Data  
 1 Symbol Tnc  
Symbol name tenascin C  
Chromosome 4  
Common name Tnc; tenascin C, Hxb, Ten, TN-C, AI528729  
Symbol description No Data  
 2 Symbol lacZ  
Symbol name beta-galactosidase (E. coli)  
Chromosome 4  
Common name No Data  
Symbol description E. coli beta galactosidase  
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)  
 3 Symbol neo  
Symbol name neomycin resistance gene (E. coli)  
Chromosome 4  
Common name neo; neomycin;  
Symbol description No Data  
References Am J Pathol. 1998 May;152(5):1237-45.
Biochem Biophys Res Commun. 1996 Apr 5;221(1):151-6.
Biochem Biophys Res Commun. 1997 Feb 13;231(2):356-9.
Biochem Biophys Res Commun. 2002 Feb 1;290(4):1220-7.
Exp Mol Pathol. 2002 Jun;72(3):186-95.
Gene. 1991 Aug 15;104(2):177-85.
Genes Dev. 1992 Oct;6(10):1821-31.
Invest Ophthalmol Vis Sci. 1999 May;40(6):1071-80.
J Comp Neurol. 2001 Feb 19;430(4):485-500.
J Invest Dermatol. 1998 Dec;111(6):930-5.
J Neurosci. 1995 Mar;15(3 Pt 1):1971-83.
Jpn J Cancer Res. 2002 Sep;93(9):968-75.
Perspect Dev Neurobiol 1994;2(1):111-16.  













Research applications Neurobiology Research  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Am. J. Pathol., 152, 1237-1245 (1998). J. Invest. Dermatol., 111, 930-935 (1998). Invest. Ophthalmol. Visual. Sci., 1071-1080 (1999).  
Additional information
 1 Genetic Background  
 2 Genotyping protocol <PCR>  
Depositor RIKEN, BioResource Center   
Strain Status /
Availability
(Expected delivery)

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