Strain Data Sheet
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Data update: Feb 22, 2017
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RBRC No. RBRC00398  
Type Targeted MutationCartagena   wks
Species Mus musculus  
Strain name GelA KO  
Former Common name GelA KO (Nov. 2011), B6.129P2-Mmp2tm1Ito  
H-2 Haplotype No Data  
ES cell line E14 [129P2/OlaHsd]  
Donor strain 129P2/OlaHsd via E14 ES cell line  
Background strain C57BL/6J Jcl  
Appearance
 1 Appearance black  
Genotype No Data  
Strain development Developed by Takeshi Itoh and Shigeyoshi Itohara, Institute for Virus Research, Kyoto University in 1997. The mutant mice were backcrossed to C57BL/6.  
Strain description This strain was generated by gene targeting of gelatinase A (Mmp2, metalloproteinase 2). Homozygous mutant mice are completely devoid of gelatinase A. 5.9 kb of the gene including exon 1 was replaced with a PGK-neo cassette. Homozygous mutant mice are viable and fertile, but show a subtle delay in their growth.  
Colony maintenance Homozygote x Heterozygote [or Crossing to C57BL/6JJcl]. Homozygous mutant mice are viable and fertile, but show a subtle delay in their growth.  
Health Report
Gene Details
Promoter No Data  
 1 Symbol Mmp2  
Symbol name Matrix metalloproteinase 2  
Chromosome 8  
Common name 72 KDa gelatinase, 72 KDa type III collagenase, Clg4a, GelA, gelatinase A, MMP-2  
Symbol description No Data  
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)  
 2 Symbol neo  
Symbol name neomycin resistance gene (E. coli)  
Chromosome 8  
Common name neo; neomycin;  
Symbol description No Data  
References J Biol Chem. 1997 Sep 5;272(36):22389-92.  



Research applications Cardiovascular Research,
Developmental Biology Research  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
J. Biol. Chem., 272, 22389-22392 (1997).  
Additional information
 1 Lab HP  
 2 Genetic Background  
 3 Genotyping protocol <PCR>  
Depositor Itohara, ShigeYoshi (RIKEN Brain Science Institute)  Itohara, ShigeYoshi 
Strain Status /
Availability
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