|
RBRC No. |
RBRC00398
|
|
Type |
Targeted Mutation |
 |
|
Species |
Mus musculus |
|
Strain name |
GelA KO |
|
Former Common name |
GelA KO (Nov. 2011), B6.129P2-Mmp2tm1Ito |
|
H-2 Haplotype |
No Data |
|
ES cell line |
E14 [129P2/OlaHsd] |
|
Donor strain |
129P2/OlaHsd via E14 ES cell line |
|
Background strain |
C57BL/6J Jcl |
|
Appearance |
| 1 |
Appearance |
black |
| Genotype |
No Data |
|
Strain development |
Developed by Takeshi Itoh and Shigeyoshi Itohara, Institute for Virus Research, Kyoto University in 1997. The mutant mice were backcrossed to C57BL/6. |
|
Strain description |
This strain was generated by gene targeting of gelatinase A (Mmp2, metalloproteinase 2). Homozygous mutant mice are completely devoid of gelatinase A. 5.9 kb of the gene including exon 1 was replaced with a PGK-neo cassette. Homozygous mutant mice are viable and fertile, but show a subtle delay in their growth. |
|
Colony maintenance |
Homozygote x Heterozygote [or Crossing to C57BL/6JJcl]. Homozygous mutant mice are viable and fertile, but show a subtle delay in their growth. |
|
Health Report |
|
|
Gene Details |
|
Promoter |
No Data |
| 1 |
Symbol |
Mmp2 |
| Symbol name |
Matrix metalloproteinase 2 |
| Chromosome |
8 |
| Common name |
72 KDa gelatinase, 72 KDa type III collagenase, Clg4a, GelA, gelatinase A, MMP-2 |
| Symbol description |
No Data |
|
Promoter |
mouse phosphoglycerate kinase promoter (PGK promoter) |
| 2 |
Symbol |
neo |
| Symbol name |
neomycin resistance gene (E. coli) |
| Chromosome |
8 |
| Common name |
neo; neomycin; |
| Symbol description |
No Data |
|
References |
J Biol Chem. 1997 Sep 5;272(36):22389-92.
|
|
Research applications |
Cardiovascular Research,
Developmental Biology Research |
|
Specific Term and Conditions |
The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
J. Biol. Chem., 272, 22389-22392 (1997). |
|
Additional information |
|
1 |
Lab HP |
|
2 |
Genetic Background |
|
3 |
Genotyping protocol <PCR> |
|
Depositor |
Itohara, ShigeYoshi (RIKEN Center for Brain Science)  |
|
Strain Status /
Availability
(Expected delivery) |
|
