Strain Data Sheet
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Data update: May 23, 2017
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RBRC No. RBRC00437  
Type Targeted Mutation CongenicCartagena   wks
Species Mus musculus  
Strain name B6.129P2-Prnp<tm1Ito>/Rbrc  
Former Common name Prnp-/- mouse, Prion protein (PrP) Knockout mice  
H-2 Haplotype No Data  
ES cell line E14 [129P2/OlaHsd]  
Donor strain 129P2/OlaHsd via E14 ES cell line  
Background strain C57BL/6J  
Appearance
 1 Appearance black  
Genotype a/a B/B C/C  
Strain development Developed by Drs. Takashi Yokoyama, National Institute of Animal Health and Shigeyoshi Itohara, RIKEN BSI in 2001.
C57BL/6J background.  
Strain description PrP, the prion protein, plays a central role in the pathogenesis of transmissible neurodegenerative disorders such as scrapie or BSE. Homozygous mutant mice show the absence of cellular isoform of prion protein. Aged homozygous mice exhibit tremor and ataxia.  
Colony maintenance Sibling Mating (Homozygote x Homozygote)
Aged mice exhibit tremor and ataxia.  
Health Report No Data  
Gene Details
Promoter No Data  
 1 Symbol PrnP  
Symbol name prion protein  
Chromosome 2  
Common name PrP, PrPC, Sinc, +Prnp, CD230, PrPSc, Prn-i, Prn-p, PrP  
Symbol description No Data  
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)  
 2 Symbol neo  
Symbol name neomycin resistance gene (E. coli)  
Chromosome 2  
Common name neo; neomycin;  
Symbol description No Data  
References J Biol Chem. 2001 Apr 6;276(14):11265-71. Epub 2001 Jan 10.
Nature. 1999 Jul 15;400(6741):225-6.  
In vivo conversion of cellular prion protein to pathogenic isoforms, as monitored by conformation-specific antibodies.
In vivo conversion of cellular prion protein to pathogenic isoforms, as monitored by conformation-specific antibodies.
Research applications Mouse Models for Human Disease  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Nature. 1999 Jul 15;400(6741):225-6.  
Additional information
 1 Genetic Background  
 2 Genotyping protocol <PCR>  
Depositor Onodera, Takashi (The University of Tokyo)  Onodera, Takashi 
Strain Status /
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