Strain Data Sheet
ANIMAL SEARCH SYSTEM
Data update: Jul 24, 2017
Health Report   PCR Protocol   Thawing Method
embryos / sperm >
  Mouse Marking
Figure
  Order
RBRC No. RBRC00445  
Type TransgeneCartagena  
Species Mus musculus  
Strain name C57BL/6-Tg(CAG/Acr-EGFP)C3-N01-FJ002Osb  
Former Common name C57BL/6-TgN(acro/act-EGFP)OsbC3-N01-FJ002  
H-2 Haplotype No Data  
Background strain C57BL/6Cr Slc  
Appearance
 1 Appearance black  
Genotype a/a B/B C/C  
Strain development Developed by Masaru Okabe, Research Institute for Microbial Diseases, Osaka University. The CAG and acr-EGFP plasmids were co-injected into the pronuclei of C57BL/6 fertilized eggs. C57BL/6NCr background.  
Strain description Transgenic mice expressing GFP ubiquitously from CAG-EGFP and in the sperm acrosome from acr3-EGFP. Acr3-EGFP, in which EGFP with a proacrosin signal peptide and proacrosin N-terminal peptide were connected to the acrosin promoter. CAG-EGFP, in which EGFP is connected to the cytomegalovirus immediate early enhancer/beta-actin promoter (CAG).  
Colony maintenance Carrier x Carrier (Homozygote x Homozygote) [or Crossing to C57BL/6NCrSlc].  
Health Report
Gene Details
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)  
 1 Symbol GFP  
Symbol name Green Fluorescent Protein (Jellyfish)  
Chromosome UN  
Common name No Data  
Symbol description No Data  
Promoter mouse proacrosin promoter, mouse proacrosin signal peptide, acrosin N-terminal peptide  
 2 Symbol GFP  
Symbol name Green Fluorescent Protein (Jellyfish)  
Chromosome UN  
Common name No Data  
Symbol description No Data  
References Curr Top Dev Biol. 1999;44:1-20.
Dev Biol. 2001 Sep 1;237(1):222-31.
FEBS Lett. 1997 May 5;407(3):313-9.
FEBS Lett. 1999 Apr 23;449(2-3):277-83.  




Research applications Fluorescent Proteins/lacZ System,
Reproductive Biology Research  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Dev. Biol., 237, 222-231 (2001).
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (http://www.uic.osaka-u.ac.jp/en/index.html). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.  
Additional information
 1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Lab HP  
 2 Genetic Background  
 3 Genotyping protocol <PCR>  
Depositor Okabe, Masaru (Osaka university)  Okabe, Masaru 
Strain Status /
Availability
(Expected delivery)

back Back
RIKEN BioResource Center

English page click here ANIMAL SEARCH SYSTEM