Strain Data Sheet
ANIMAL SEARCH SYSTEM
Data update: Jan 30, 2017
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RBRC No. RBRC01071  
Type Targeted MutationCartagena   wks
Species Mus musculus  
Strain name Floxed RBP-J  
Former Common name No Data  
H-2 Haplotype No Data  
ES cell line E14 [129P2/OlaHsd]  
Donor strain Not Specified  
Background strain No Data  
Appearance
 1 Appearance black  
Genotype a/a B/B C/C  
Strain development Developed by Tasuku Honjo, Graduate School of Medicine, Kyoto University. The construct was electoroporated into E14 ES cells derived from 129P2/OlaHsd. The mutant mice were crossed to C57BL/6.  
Strain description Rbpj floxed mice. Rbpj, recombination signal binding protein for immunoglobulin kappa J region is a transcriptional factor of a Notch/RBP-J signaling pathway play a role in fate determination in various lineages. Exon 6 and 7 of Rbpj gene was flanked by loxP sites, and a neomycin resistance gene was inserted between exon 7 and the loxP site. Conditional RBP-J deficient mice can be generated by crossing with tissue-specific Cre mice.  
Colony maintenance Homozygote x Homozygote. Homozygous mice are viable and fertile.  
Health Report
Gene Details
Promoter No Data  
 1 Symbol Rbpj  
Symbol name recombining binding protein suppressor of hairless (Drosophila)  
Chromosome 5  
Common name CBF1, Igkjrb, Igkrsbp, RBP-J, RBP-J kappa, RBPjk, Rbpsuh  
Symbol description No Data  
 2 Symbol loxP  
Symbol name phage P1 loxP  
Chromosome 5  
Common name No Data  
Symbol description No Data  
 3 Symbol loxP  
Symbol name phage P1 loxP  
Chromosome 5  
Common name No Data  
Symbol description No Data  
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)  
 4 Symbol neo  
Symbol name neomycin resistance gene (E. coli)  
Chromosome 5  
Common name neo; neomycin;  
Symbol description No Data  
References Int Immunol. 2002 Jun;14(6):637-45.  
Notch signaling controls liver development by regulating biliary differentiation.
Notch signaling controls liver development by regulating biliary differentiation.
Research applications Cre/loxP system  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Int, Immunol., 14, 637-645 (2002).
1) A material transfer agreement specified by DEPOSITOR and RIKEN BRC will be used for the transfer of the BIOLOGICAL RESOURCE from RIKEN BRC to the RECIPIENT.
2) The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results.
3) Upon completion or discontinuation of the research specified in the MTA , the RECIPIENT shall promptly report to the DEPOSITOR any results from its research using the BIOLOGICAL RESOURCE (hereinafter "RESEARCH RESULT").
4) In publishing the research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature designated in the appendix by the DEPOSITOR is required.
5) The RECIPIENT agrees that the DEPOSITOR and his collaborators on PD-1
research shall be free to use the RESEARCH RESULT for research purpose
without any compensation.
6) The RECIPIENT shall not transfer, sell or assign the BIOLOGICAL
RESOURCE or any part thereof, including without limitation those contained in
modification or cross-bred mouse, to any third party.
7) RECIPIENT shall notify the PROVIDER upon filing a patent application claiming modification of the BIOLOGICAL RESOURCE or method(s) of manufacture or use(s) of the BIOLOGICAL RESOURCE.  
Additional information
 1 MATERIAL TRANSFER AGREEMENT (Japanese / English)
Honjo Lab HP  
 2 Genetic Background  
 3 Genotyping protocol <PCR>  
Depositor Honjo, Tasuku (Graduate School of Medicine, Kyoto University)  Honjo, Tasuku 
Strain Status /
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