Strain Data Sheet
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Data update: Jan 30, 2017
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RBRC No. RBRC01145  
Type Targeted MutationCartagena  
Species Mus musculus  
Strain name ICR.Cg-Mesp1<tm2(cre)Ysa>/YsaRbrc  
Former Common name Mesp1-cre  
H-2 Haplotype No Data  
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]  
Donor strain (C57BL/6 x CBA)F1 via TT2 ES cell line  
Background strain ICR MCH  
Appearance
 1 Appearance Agouti or black  
Genotype A/? or a/a B/B Mesp1-Cre c/+ C  
 2 Appearance albino  
Genotype A/? or a/a B/B + c/+ C  
Strain development Developed by Yumiko Sga, National Institute of Health Sciences in 1998. A Cre gene and pgk-neo cassette were ligated in frame at a NcoI site located at the translational initiation site of Mesp1 gene. ICR mixed background.  
Strain description Mesp1-Cre knock-in mice, in which Cre recombinase is expressed under the control of the endogenous promoter-enhancer. By crossing with a mouse strain with a reporter gene like a GFP and LacZ which is interrupted by a floxed CAT gene, it is possible to detect Mesp1-expressing cells. Mesp1 is a transcriptional factor temporarily expressing in endothelium of the blood vessel in a part of lateral plate mesoderm and heart cells, and is a determinant that is important for the formation of heart. This strain is useful for understanding of the mechanisms underlying cardiovascular development. Homozygous mutant mice are embryonic lethal.  
Colony maintenance Heterozygote x Wild-type [or Crossing to Jcl:MCH(ICR)]. Homozygous mutant mice are embryonic lethal.  
Health Report
Gene Details
Promoter No Data  
 1 Symbol Mesp1  
Symbol name mesoderm posterior 1  
Chromosome 7  
Common name No Data  
Symbol description No Data  
Promoter Mesp1  
 2 Symbol cre  
Symbol name Phage P1 Cre recombinase  
Chromosome 7  
Common name No Data  
Symbol description No Data  
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)  
 3 Symbol neo  
Symbol name neomycin resistance gene (E. coli)  
Chromosome 7  
Common name neo; neomycin;  
Symbol description No Data  
References Development. 1999 Aug;126(15):3437-47.  
Epiblastic Cited2 deficiency results in cardiac phenotypic heterogeneity and provides a mechanism for haploinsufficiency.
Epiblastic Cited2 deficiency results in cardiac phenotypic heterogeneity and provides a mechanism for haploinsufficiency.
Research applications Cre/loxP system,
Developmental Biology Research  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Development, 126, 3437-3447 (1999).  
Additional information
 1 Saga Lab HP  
 2 Mouse of the Month Feb 2006
Genetic Background  
 3 Genotyping protocol <PCR>  
Depositor Saga, Yumiko (National Institute of Genetics)  Saga, Yumiko 
Strain Status /
Availability
(Expected delivery)

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