Strain Data Sheet
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Data update: Oct 24, 2017
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RBRC No. RBRC01456  
Type TransgeneCartagena  
Species Mus musculus  
Strain name B6.B6D2-Tg(Neurog3-cre)24Syos  
Former Common name ngn3/Cre transgenic mouse, ngn3/Cre Tg#24  
H-2 Haplotype No Data  
Background strain C57BL/6  
Appearance
 1 Appearance black  
Genotype a/a B/B C/C  
Strain development Developed by Shosei Yoshida, Graduate School of Medicine, Kyoto University in 2001. The ngn3-Cre transgene containing a 23-kb genomic region of the mouse ngn3 locus and cre recombinase gene was injected into the pronuclei of B6D2F1 fertilized eggs. The transgenic mice were backcrossed to C57BL/6.  
Strain description These transgenic mice express a cre recombinase gene under the control of the mouse Neurogenin 3 gene promoter. This can be used to make an conditional knock-out by crossing with the other genetically-engineered mice that have floxed genes. The expression of cre recombinase was observed in positive spermatogonia, pancreatic/enteric endocrine precursors.  
Colony maintenance Carrier x Noncarrier [C57BL/6NCrSlc]. Tg mice are viable and fertile.  
Health Report
Gene Details
Promoter mouse neurogenin 3 promoter, mouse metallothionein poly A  
 1 Symbol cre  
Symbol name Phage P1 Cre recombinase  
Chromosome UN  
Common name No Data  
Symbol description No Data  
References Dev Biol 2004 May 15;269(2):447-58.  
Neurogenin3 delineates the earliest stages of spermatogenesis in the mouse testis.
Research applications Cell Biology Research,
Cre/loxP system  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Dev. Biol., 269, 447-458 (2004).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The RECIPIENT of the BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. This consent shall include the outline of the experiments in order to avoid possible conflict with the DEPOSITOR and other-workers. The RECIPIENT is prohibited to release the BIOLOGICAL RESOURCE to third parties without permission by the DEPOSITOR or use it for Experiments without prior agreement with the DEPOSITOR.  
Additional information
 1 Lab HP  
 2 Mouse of the Month July 2006
Genetic Background  
 3 Genotyping protocol <PCR>  
Depositor Yoshida, Shosei (National Institutes for Basic Research)  Yoshida, Shosei 
Strain Status /
Availability
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