Strain Data Sheet
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Data update: Jul 24, 2017
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RBRC No. RBRC01544  
Type Targeted MutationCartagena   wks
Species Mus musculus  
Strain name B6.129P2-Sirpa<tm1Nog>/Rbrc  
Former Common name SHPS-1 Knockout mice  
H-2 Haplotype No Data  
ES cell line E14.1 [129P2/OlaHsd]  
Donor strain 129P2/OlaHsd via E14.1 ES cell line  
Background strain C57BL/6  
Appearance
 1 Appearance black  
Genotype No Data  
Strain development Developed by Dr. Kenjiro Inagaki, Kobe University School of Medicine in 2000. The construct was electoroporated into E14.1 ES cells derived from 129P2/OlaHsd. The mutant mice were backcrossed C57BL/6N over 10 times.  
Strain description SHPS-1 knockout mice. Exon 7 and 8 were replaced with a nemoycin selection cassette. SHPS-1 (Ptpns1, protein tyrosine phosphatase, non-receptor type substrate 1) gene ia a member of sinal-regulatory proteins, plays a impotant roles in integrin-mediated cytoskeletal reoganization, cell motility and the regulation of Rho.  
Colony maintenance Heterozygote x Wild-type [C57BL/6NJcl]. Homozygous mutant mice are viable and fertile.  
Health Report
Gene Details
Promoter No Data  
 1 Symbol Sirpa  
Symbol name signal-regulatory protein alpha  
Chromosome 2  
Common name Bit, CD172a, P84, SHPS-1, SIRP; Ptpns1;protein tyrosine phosphatase, non-receptor type substrate 1  
Symbol description No Data  
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)  
 2 Symbol neo  
Symbol name neomycin resistance gene (E. coli)  
Chromosome 2  
Common name neo; neomycin;  
Symbol description No Data  
References EMBO J 2000 Dec 15;19(24):6721-31.  
SHPS-1 regulates integrin-mediated cytoskeletal reorganization and cell motility.
Research applications No Data  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE must submit its receipt to the DEPOSITOR immediately after receiving the BIOLOGICAL RESOURCE.  
Additional information
 1 Lab HP  
 2 Genetic Background  
 3 Genotyping protocol <PCR>  
Depositor Matozaki, Takashi (Kobe University)  Matozaki, Takashi 
Strain Status /
Availability
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