|
RBRC No. |
RBRC01578
|
|
Type |
Targeted Mutation |
 |
|
Species |
Mus musculus |
|
Strain name |
B6.Cg-Pik3r1<tm1Tka>/Rbrc |
|
Former Common name |
C57BL/6-PI3K KO |
|
H-2 Haplotype |
No Data |
|
ES cell line |
TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] |
|
Donor strain |
(C57BL/6 x CBA)F1 via TT2 ES cell line |
|
Background strain |
No Data |
|
Appearance |
| 1 |
Appearance |
black |
| Genotype |
a/a B/B C/C |
|
Strain development |
Developed by Dr. Kadowaki of the University of Tokyo and backcrossed to C57BL/6 for 12 generations by depositor. |
|
Strain description |
PI3K, phosphoinositide-3-kinase gene is a lipid kinases that catalyze the addition of a phosphate molecule specifically to the 3-position of the inositol ring of phosphoinositides. This strain is useful for elucidating of the rule of PI3K in various phases of immune responses. Homozygous mutant females were sterile. |
|
Colony maintenance |
Since most of homozygous females are sterile, we maintain the colony by mating heterozygous females with homozygous males. |
|
Health Report |
|
|
Gene Details |
|
Promoter |
No Data |
| 1 |
Symbol |
Pik3r1 |
| Symbol name |
phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha) |
| Chromosome |
13 |
| Common name |
p50alpha, p55alpha, p85alpha, PI3K |
| Symbol description |
No Data |
|
Promoter |
herpes simplex virus thymidine kinase (HSV TK) poly A |
| 2 |
Symbol |
neo |
| Symbol name |
neomycin resistance gene (E. coli) |
| Chromosome |
13 |
| Common name |
neo; neomycin; |
| Symbol description |
No Data |
|
References |
Nat Genet. 1999 Feb;21(2):230-5.
Science. 1999 Jan 15;283(5400):390-2. Increased insulin sensitivity and hypoglycaemia in mice lacking the p85 alpha subunit of phosphoinositide 3-kinase.
Increased insulin sensitivity and hypoglycaemia in mice lacking the p85 alpha subunit of phosphoinositide 3-kinase. |
|
Research applications |
No Data |
|
Specific Term and Conditions |
The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Science, 283, 390-392 (1999). Nat. genet., 21, 230-235 (1999).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. |
|
Additional information |
|
1 |
Lab HP |
|
2 |
Genotyping protocol <PCR> |
|
Depositor |
Koyasu, Shigeo (Keio University)  |
|
Strain Status /
Availability
(Expected delivery) |
|
