Strain Data Sheet
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Data update: Oct 23, 2014
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RBRC No. RBRC01578  
Type Targeted MutationCartagena  
Species Mus musculus  
Strain name C57BL/6-PI3K KO  
Former Common name No Data  
H-2 Haplotype No Data  
Donor strain (C57BL/6 x CBA)F1 via TT2 ES cell line  
Background strain No Data  
Appearance
 1 Appearance black  
Genotype a/a B/B C/C  
Strain development Developed by Dr. Kadowaki of the University of Tokyo and backcrossed to C57BL/6 for 12 generations by depositor.  
Strain description PI3K, phosphoinositide-3-kinase gene is a lipid kinases that catalyze the addition of a phosphate molecule specifically to the 3-position of the inositol ring of phosphoinositides. This strain is useful for elucidating of the rule of PI3K in various phases of immune responses. Homozygous mutant females were sterile.  
Colony maintenance Since most of homozygous females are sterile, we maintain the colony by mating heterozygous females with homozygous males.  
Health Certificate
    BRC facility Health Certificate 2013.2-2014.9
Gene Details
Promoter No Data  
 1 Symbol Pik3r1  
Symbol name phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha)  
Chromosome 13  
Common name p50alpha, p55alpha, p85alpha, PI3K  
Symbol description No Data  
Promoter herpes simplex virus thymidine kinase (HSV TK) poly A  
 2 Symbol neo  
Symbol name neomycin resistance gene (E. coli)  
Chromosome 13  
Common name neo; neomycin;  
Symbol description No Data  
References Nat Genet. 1999 Feb;21(2):230-5.
Science. 1999 Jan 15;283(5400):390-2.  
Increased insulin sensitivity and hypoglycaemia in mice lacking the p85 alpha subunit of phosphoinositide 3-kinase.
Increased insulin sensitivity and hypoglycaemia in mice lacking the p85 alpha subunit of phosphoinositide 3-kinase.
Research applications No Data  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Science, 283, 390-392 (1999). Nat. genet., 21, 230-235 (1999).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.  
Additional information
 1 Lab HP  
 2 Genotyping protocol <PCR>  
Depositor Koyasu, Shigeo (Keio University)  Koyasu, Shigeo 
Strain Status /
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