Strain Data Sheet
ANIMAL SEARCH SYSTEM
Data update: May 23, 2017
Health Report   PCR Protocol   Thawing Method
embryos / sperm >
  Mouse Marking
Figure
  Order
RBRC No. RBRC02095  
Type Transgene CongenicCartagena  
Species Mus musculus  
Strain name B6.B6D2-Tg(Th-EGFP)21-31Koba  
Former Common name B6.Cg-Tg(TH-GFP)21-31  
H-2 Haplotype No Data  
Background strain C57BL/6  
Appearance
 1 Appearance black  
Genotype a/a B/B C/C  
Strain development Developed by Kazuto Kobayashi, Fukushima Medical University School of Medicine in 1998. The transgene was injected into the pronuclei of B6D2F2 fertilized eggs. The transgenic mice were backcrossed to C57BL/6J. Two lines were generated. RBRC02095 (commonly-used), RBRC03162.  
Strain description The TH-GFP transgenic mouse was generated to express GFP in the majority of midbrain dopamine neurons under the control of the rat TH gene promoter. Dopamine is a neurotransmitter which mediates motor control, cognition, emotion, reward, and neuroendocrine functions. Dysfunction of dopamine-producing neurons in the midbrain is involved in some neurological and neuropsychiatric diseases such as Parkinson's disease and schizophrenia. These dopamine neurons express tyrosine hydroxylase (TH), a key enzyme of catecholamine biosynthesis. This transgenic mouse is useful for visualizing dopamine neurons to study the physiology and pathogenesis of dopamine neurons. Homozygous transgenic mice are lethal.  
Colony maintenance Carrier x Noncarrier [C57BL/6JJcl]  
Health Report
Gene Details
Promoter rat tyrosine hydroxylase (TH) promoter  
 1 Symbol GFP  
Symbol name Green Fluorescent Protein (Jellyfish)  
Chromosome UN  
Common name No Data  
Symbol description No Data  
References J Neurochem. 2002 Jul;82(2):295-304.  
Dynamics of tyrosine hydroxylase promoter activity during midbrain dopaminergic neuron development.
Research applications Fluorescent Proteins/lacZ System,
Neurobiology Research  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
J. Neurochem., 82, 295-304 (2002).
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.  
Additional information
 1 Construct map
Transgene sequence  
 2 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Lab HP (Japanese)  
 3 Mouse of the Month May 2008
Genetic Background  
 4 Genotyping protocol <PCR>  
Depositor Kobayashi, Kazuto (Fukushima Medical University)  Kobayashi, Kazuto 
Strain Status /
Availability
(Expected delivery)

back Back
RIKEN BioResource Center

English page click here ANIMAL SEARCH SYSTEM