Strain Data Sheet
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Data update: Nov 22, 2017
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RBRC No. RBRC02142  
Type Targeted Mutation CongenicCartagena   wks
Species Mus musculus  
Strain name B6.129S2-Pdcd1<tm1Hon>/HonRbrc  
Former Common name C57BL/6-PD-1KO, C57BL/6-Pdcd1-/- mouse  
H-2 Haplotype No Data  
ES cell line D3 [129S2/SvPas]  
Background strain C57BL/6  
Appearance
 1 Appearance black  
Genotype (a/a B/B C/C)  
Strain development Developed by Tasuku Honjo, Graduate School of Medicine, Kyoto University in 1998. The knockout construct was electoroporated into D3 ES cells derived from 129S2/SvPas. The mutant mice were backcrossed to C57BL/6 over 11 generations.  
Strain description PD1 knockout mice. EcoRV site of exon 3 to PvuII site of exon 5 was replaced with noemycin selection cassette. PD1 (Pdcd1, programmed cell death 1) gene is an immunoinhibitory receptor that belongs to the CD28 family, plays a role in the negative control of proliferation, differentiation and class switching of B cells. PD1 deficiency has been shown to develop different forms of autoimmune diseases on different backgrounds of mice. Homozygous PD1 mutant mice of C57BL/6 background show moderate but consistent splenomegaly, and spontaneously develop characteristic lupus-like proliferative arthritis and golmerulonephritis with the age.  
Colony maintenance Homozygote x Homozygote.  
Health Report
Gene Details
Promoter No Data  
 1 Symbol Pdcd1  
Symbol name programmed cell death 1  
Chromosome 1  
Common name Ly101, PD-1, Pdc1  
Symbol description No Data  
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)  
 2 Symbol neo  
Symbol name neomycin resistance gene (E. coli)  
Chromosome 1  
Common name neo; neomycin;  
Symbol description No Data  
References Immunity. 1999 Aug;11(2):141-51.
Int Immunol. 1998 Oct;10(10):1563-72.  
Development of lupus-like autoimmune diseases by disruption of the PD-1 gene encoding an ITIM motif-carrying immunoreceptor.
Development of lupus-like autoimmune diseases by disruption of the PD-1 gene encoding an ITIM motif-carrying immunoreceptor.
Research applications Immunology and Inflammation Research  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
1) The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results.
2) Upon completion or discontinuation of the research specified in the MTA , the RECIPIENT shall promptly report to the DEPOSITOR any results from its research using the BIOLOGICAL RESOURCE (hereinafter "RESEARCH RESULT").
3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. .Science 291, 319-322 (2001)
4) The RECIPIENT agrees that the DEPOSITOR and his collaborators on PD-1 research shall be free to use the RESEARCH RESULT for research purpose
without any compensation.
5) RECIPIENT shall notify the PROVIDER upon filing a patent application claiming modification of the BIOLOGICAL RESOURCE or method(s) of manufacture or use(s) of the BIOLOGICAL RESOURCE.  
Additional information
 1 Honjo Lab HP  
 2 Mouse of the Month Jul 2012
Genetic Background
Breeding characters  
 3 Genotyping protocol <PCR>  
Depositor Honjo, Tasuku (Graduate School of Medicine, Kyoto University)  Honjo, Tasuku 
Strain Status /
Availability
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