Strain Data Sheet
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Data update: Feb 22, 2017
Health Report   PCR Protocol   Thawing Method
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RBRC No. RBRC02231  
Type Targeted MutationCartagena  
Species Mus musculus  
Strain name B6;129-Atg5<tm1Nmz>  
Former Common name No Data  
H-2 Haplotype No Data  
ES cell line R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]  
Background strain No Data  
Appearance
 1 Appearance black  
Genotype a/a B/B C/C  
Strain development Developed by Masahiko Hatano and Takeshi Tokuhisa, Graduate School of Medicine, Chiba University, and Noboru Mizushima, National Institute for Basic Biology in 2003. The knockout construct was electoroporated into R1 ES cells derived from (129X1/SvJ x 129S1/Sv)F1-Kitl<+>. The mutant mice were backcrossed to C57BL/6J.  
Strain description Atg5 knockout mice. Exons 1 and 2 of Atg5 were replaced with a neomycin resistance gene. Homozygous mutant mice die during the early neonatal period due to a lack of nutrients. Autophagy is an intracellular degradation process by an autophagosome, which contains a portion of cytoplasm and subsequently degrades upon fusion with a lysosome. Autophagy is considered to be important for the cellular response to starvation and the normal turnover of cytoplasmic components as well. Atg5, autophagy-related 5 is essential for autophagosome formation. Conditional mice (floxed allele) are also available: B6.129S-Atg5<tm1Myok> (RBRC02975).  
Colony maintenance Heterozygote x Wild-type [C57BL/6JJmsSlc]  
Health Report
Gene Details
Promoter No Data  
 1 Symbol Atg5  
Symbol name autophagy-related 5 (yeast)  
Chromosome 10  
Common name 2010107M05Rik, 3110067M24Rik, Apg5l, Paddy  
Symbol description No Data  
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)  
 2 Symbol neo  
Symbol name neomycin resistance gene (E. coli)  
Chromosome 10  
Common name neo; neomycin;  
Symbol description No Data  
References Nature. 2004 Dec 23;432(7020):1032-6. Epub 2004 Nov 3.  
The role of autophagy during the early neonatal starvation period.
Research applications Cell Biology Research  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Nature, 432, 1032-1036 (2004).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
BIOLOGICAL RESOURCE is limited to for academic research in non-profit organization. Any use of the BIOLOGICAL RESOURCE for profit purposes by a non-profit organization or any use of the BIOLOGICAL RESOURCE by a profit organization requires a separate license from the DEPOSITOR prior to distribution. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.  
Additional information
 1 Mouse of the Month Jul 2009
Genetic Background  
 2 Lab HP  
 3 Genotyping protocol <PCR>  
Depositor Mizushima, Noboru (The University of Tokyo, Graduate School of Medicine)  Mizushima, Noboru 
Strain Status /
Availability
(Expected delivery)

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