Strain Data Sheet
ANIMAL SEARCH SYSTEM
Data update: Aug 23, 2017
Health Report   PCR Protocol   Thawing Method
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RBRC No. RBRC02302  
Type Targeted MutationCartagena   wks
Species Mus musculus  
Strain name B6.129P2-Lyzs<tm1(cre)Ifo>  
Former Common name LysM-Cre knock-in mice (C57BL/6)  
H-2 Haplotype No Data  
ES cell line E14 [129P2/OlaHsd]  
Background strain C57BL/6  
Appearance
 1 Appearance black  
Genotype a/a B/B C/C  
Strain development Developed by Technical University of Munich, Irmgard Foester. Deposited by Shigeo Koyasu, School of Medicine, Keio University through Akita University Graduate School of Medicine. E14 ES cells were used to generate the knock-in mice. The mice were crossed to C57BL/6 for several times, and then backcrossed to C57BL/6NTac and BALB/cAnN, respectively (RBRC02302 and RBRC02303).  
Strain description Lyzs (Lyz2, lysozyme 2) Cre knock-in mice, known as the LysMcre mouse. A NLS-cre cDNA was inserted into the endogenous ATG start site of Lyz2 gene. LysMcre mice allow for both specific and highly efficient Cre-mediated deletion of loxP-flanked target genes in myeloid cells. C57BL/6 background (RBRC02302) and BALB/c background (RBRC02303).  
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6NTac]  
Health Report
Gene Details
Promoter No Data  
 1 Symbol Lyzs  
Symbol name lysozyme  
Chromosome 10  
Common name LC, Lys-cre, LysM Cre, lysM/cre, LysMCre, LysMcre  
Symbol description No Data  
 2 Symbol FRT  
Symbol name yeast FRT (flippase recombination target) site  
Chromosome 10  
Common name No Data  
Symbol description No Data  
 3 Symbol cre  
Symbol name Phage P1 Cre recombinase  
Chromosome 10  
Common name No Data  
Symbol description No Data  
 4 Symbol nls  
Symbol name SV40 large T antigen nuclear localization signal (NLS)  
Chromosome 10  
Common name No Data  
Symbol description No Data  
References   
Research applications Cell Biology Research,
Cre/loxP system,
FLP/frt system  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOER. Dr. Irmgard Foester, Life and Medical Sciences (LIMES) Institute, University of Bonn E-mail: irmgard.foerster@uni-bonn.de  
Additional information
 1 Lab HP  
 2 Genetic Background  
 3 Genotyping protocol <PCR>  
Depositor Koyasu, Shigeo (Keio University)  Koyasu, Shigeo 
Strain Status /
Availability
(Expected delivery)

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