Strain Data Sheet
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Data update: Jul 24, 2017
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RBRC No. RBRC02705  
Type Transgene CongenicCartagena  
Species Mus musculus  
Strain name B6.Cg-Tg(FucciS/G2/M)#492Bsi  
Former Common name B6.Cg-Tg(Fucci)492Bsi, B6;B6D2-Tg(Fucci)492Bsi  
H-2 Haplotype No Data  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Asako Sakaue-Sawano and Atsushi Miyawaki, RIKEN Brain Science Institute. The transgene was injected into the pronuclei of B6D2F1 fertilized eggs. The mice were backcrossed to C57BL/6N.  
Strain description Transgenic mouse strains express fucci (fluorescent, ubiquitination-based cell cycle indicator) under the control of CAG promoter. These mice can be used to clearly visualize the cell cycle with fluorescence, i.e. S/G2/M phase nuclei green and those in G1 phase red in vivo. #474 (RBRC02704), #492 (RBRC02705), #504 (RBRC02706): Fucci-S/G2/M-Green (mAG-hGeminin(1/110)). #596 (RBRC02707), #610 (RBRC02708), #639 (RBRC02709): Fucci-G1-Red (mKO2-hCdt1(30/120)). For whole body (various tissues): #504 and #596 (Cell 2008). For hematopoietic system (monocyte and macrophage): #474 and #610 (Development 2013). For lymphocytic system: #492 and 639 (PNAS 2010). #474 and #610 double transgenic mice (RBRC02817). #504 and #596 double transgenic mice (RBRC02892). Whole-body visualization of Fucci mice with a flashlight (PCR genotyping is also available.): Check the Fucci S/2G/M-Green expressing mice at 1-4 days after birth by using blue LED flashlight through a sharp-cut filter in the dark box. Check the Fucci G1-Red expressing mice within 1-4 days after birth by using green LED flashlight through a green band-cut filter in the dark box. The part with few hairs such as in the mouth and auricles is suitable for the observation of fluorescence. Visualizing device: blue LED flashlight (470 nm), S470/30 filter, 535AF26 filter, sharp-cut filter (500 or 520 nm), green band-cut filter (red filter). #474 and #504: Tg homozygous mice are viable and fertile. #596: Tg homozygous mice are probably lethal (details unknown). #492, #610 and #639: Viability and fertility of homozygous mice are unknown (probably decreased fertility, details unknown).  
Colony maintenance Carrier x Noncarrier [C57BL/6NJcl]  
Health Report
Gene Details
Promoter No Data  
 1 Symbol GMNN  
Symbol name geminin (human)  
Chromosome UN  
Common name No Data  
Symbol description No Data  
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)  
 2 Symbol mAG  
Symbol name monomeric Azami Green (Coral)  
Chromosome UN  
Common name No Data  
Symbol description No Data  
References Cell. 2008 Feb 8;132(3):487-98.
  
Visualizing spatiotemporal dynamics of multicellular cell-cycle progression.
Visualizing spatiotemporal dynamics of multicellular cell-cycle progression.
Research applications Fluorescent Proteins/lacZ System  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. The RECIPIENT of the BIOLOGICAL RESOURCE is requested to make a Material Transfer Agreement regarding the usage of the CAG promoter specified by the DEVELOPER, Dr. Junichi Miyazaki. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a non-for profit institution for a not-for-profit research. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature (Proc. Natl. Acad. Sci. U S A., 107 (27): 12192-12197 (2010) designated by the DEPOSITOR is requested. The RECIPIENT agrees to acknowledge PROVIDER in all oral presentations and written publications as related to the MATERIAL and provide a copy of the publication to the DEPOSITER.  
Additional information
 1 Genetic Background  
 2 CAGGS MATERIAL TRANSFER AGREEMENT (Pdf)/ (Word)
Miyawaki Lab HP  
 3 Genotyping protocol <PCR>  
Depositor Miyawaki, Atsushi (RIKEN Brain Science Institute)  Miyawaki, Atsushi 
Strain Status /
Availability
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