Strain Data Sheet
ANIMAL SEARCH SYSTEM
Data update: Jul 24, 2017
Health Report   PCR Protocol   Thawing Method
embryos / sperm >
  Mouse Marking
Figure
  Order
RBRC No. RBRC02708  
Type Transgene CongenicCartagena  
Species Mus musculus  
Strain name B6.Cg-Tg(FucciG1)#610Bsi  
Former Common name B6.Cg-Tg(Fucci)610Bsi, B6;B6D2-Tg(Fucci)610Bsi  
H-2 Haplotype No Data  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Asako Sakaue-Sawano and Atsushi Miyawaki, RIKEN Brain Science Institute. The transgene was injected into the pronuclei of B6D2F1 fertilized eggs. The mice were backcrossed to C57BL/6N.  
Strain description Transgenic mouse strains express fucci (fluorescent, ubiquitination-based cell cycle indicator) under the control of CAG promoter. These mice can be used to clearly visualize the cell cycle with fluorescence, i.e. S/G2/M phase nuclei green and those in G1 phase red in vivo. #474 (RBRC02704), #492 (RBRC02705), #504 (RBRC02706): Fucci-S/G2/M-Green (mAG-hGeminin(1/110)). #596 (RBRC02707), #610 (RBRC02708), #639 (RBRC02709): Fucci-G1-Red (mKO2-hCdt1(30/120)). For whole body (various tissues): #504 and #596 (Cell 2008). For hematopoietic system (monocyte and macrophage): #474 and #610 (Development 2013). For lymphocytic system: #492 and 639 (PNAS 2010). #474 and #610 double transgenic mice (RBRC02817). #504 and #596 double transgenic mice (RBRC02892). Whole-body visualization of Fucci mice with a flashlight (PCR genotyping is also available.): Check the Fucci S/2G/M-Green expressing mice at 1-4 days after birth by using blue LED flashlight through a sharp-cut filter in the dark box. Check the Fucci G1-Red expressing mice within 1-4 days after birth by using green LED flashlight through a green band-cut filter in the dark box. The part with few hairs such as in the mouth and auricles is suitable for the observation of fluorescence. Visualizing device: blue LED flashlight (470 nm), S470/30 filter, 535AF26 filter, sharp-cut filter (500 or 520 nm), green band-cut filter (red filter). #474 and #504: Tg homozygous mice are viable and fertile. #596: Tg homozygous mice are probably lethal (details unknown). #492, #610 and #639: Viability and fertility of homozygous mice are unknown (probably decreased fertility, details unknown).  
Colony maintenance Carrier x Noncarrier [C57BL/6NJcl]  
Health Report
Gene Details
Promoter No Data  
 1 Symbol CDT1  
Symbol name chromatin licensing and DNA replication factor (human)  
Chromosome UN  
Common name No Data  
Symbol description No Data  
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)  
 2 Symbol mKO2  
Symbol name monomeric Kusabira Orange (Coral)  
Chromosome UN  
Common name No Data  
Symbol description No Data  
References Cell. 2008 Feb 8;132(3):487-98.
  
Visualizing spatiotemporal dynamics of multicellular cell-cycle progression.
Visualizing spatiotemporal dynamics of multicellular cell-cycle progression.
Research applications Fluorescent Proteins/lacZ System  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Development (2013) doi:10.1242/dev.099226
The RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. The RECIPIENT agrees to acknowledge PROVIDER in all oral presentations and written publications as related to the MATERIAL and provide a copy of the publication to the DEPOSITER.  
Additional information
 1 Genetic Background  
 2 CAGGS MATERIAL TRANSFER AGREEMENT (Pdf)/ (Word)
Miyawaki Lab HP  
 3 Genotyping protocol <PCR>  
Depositor Miyawaki, Atsushi (RIKEN Brain Science Institute)  Miyawaki, Atsushi 
Strain Status /
Availability
(Expected delivery)

back Back
RIKEN BioResource Center

English page click here ANIMAL SEARCH SYSTEM