Strain Information | |
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Image | |
BRC No. | RBRC05551 |
Type | Targeted Mutation and Transgene |
Species | Mus musculus |
Strain name | B6.Cg-Tg(Aicda-cre)9Mbu Gt(ROSA)26Sor<tm1Hjf> |
Former Common name | Aicda-cre/rosa-tdRFP, B6-AicdaCre/ROSA26-tdRFP |
H-2 Haplotype | |
ES Cell line | AK7 [129S4/SvJaeSor] |
Background strain | |
Appearance | |
Strain development | Developed by Hitoshi Nagaoka, Graduate School of Medicine, Kyoto University. Rosa-tdRFP : Hans Jorg Fehling, Institute of Immunology, University Clinics Ulm. Aicda-cre : Meinrad Busslinger. Research Institute of Molecular Pathology. |
Strain description | This strain has AID-Tg (Tg(Aicda-cre)9Mbu) and ROSA26 tdRFP reporter (Gt(ROSA)26Sor<tm1Hjf>) alleles. |
Colony maintenance | Tg : Carrier x Carrier ; ROSA26 : Homozygote x Homozygote |
References | Immunity, 28, 751-762 (2008). 18538592 PLoS One, 6, e29141 (2011). 22216188 Eur. J. Immunol., 37, 43-53 (2007). 17171761 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1Hjf>targeted mutation 1, Hans Jorg Fehling Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter GHGrowth hormone polyA (Bovine)6GH Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter RFPRed Fluorescent Protein (Discosoma sp.)6RFP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SASplice acceptor (adenovirus)6SA Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lox2272phage P1 loxP variant6lox2272 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lox2272phage P1 loxP variant6lox2272 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter CD2CD2 (human)UNCD2 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Iresinternal ribosomal entry site (EMCV)UN Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter crePhage P1 Cre recombinaseUNcremouse Aicda promoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter nlsSV40 large T antigen nuclear localization signal (NLS)UNnls Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 polyA signalUN |
Ordering Information | |
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Donor DNA | mouse Aicda genomic DNA (BAC clone RP24-68I7), SV40 nuclear localization sequence, Phage P1 Cre, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), human CD2 cDNA, SV40 polyadenylation site, yeast FRT (flipase recombination target) site, adenovirus splice acceptor site (SA), P1 phage loxP sites, E. coli neo, bovine growth hormone gene polyadenylation site, Discosoma sp. tdRFP cDNA, SV40 polyadenylation site, mouse ROSA26 locus genomic DNA |
Research application | Fluorescent Proteins/lacZ System |
Specific Term and Conditions | 1) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested. PLoS One. 2011 6(12):e29141. 2) The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results. 3) The BIOLOGICAL RESOURCE contains Tg(Aicda-cre)9Mbu and Gt(ROSA)26Sortm1Hjf. Before executing the MTA between RIKEN BRC andthe RECIPIENT, the RECIPIENT shall obtain the prior written consent from two developer scientists; Dr. Meinrad Busslinger(Research Institute of Molecular Pathology) to use Tg(Aicda-cre)9Mbu and Dr. Hans Joerg Fehling, Institute of Immunology, University Clinics Ulm to use Gt(ROSA)26Sortm1Hjf. |
Depositor | Tasuku Honjo (Kyoto University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Genotyping protocol -PCR- Necessary documents for ordering:
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BRC mice in Publications |
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