Strain Data Sheet

RBRC06258

Strain Information

Image
BRC No.RBRC06258
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(Cacng5-EGFP)500Bsi
Former Common nameCacng5 promoter-GFP, B6-Cacng5-GFP Tg#500
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Yoshiaki Shinohara and Hajime Hirose, RIKEN Brain Science Institute. The BAC transgenic construct containing the mouse Cacng5 gene with IRES-GFP (GENSAT1-BX1300) was used. C57BL/6 background.
Strain descriptionhese transgenic mice express IRES-GFP under the control of the mouse Cacng5 promoter.
Colony maintenanceCarrier x Noncarrier [C57BL/6JJmsSlc]
ReferencesEur. J. Neurosci., 35, 702-710 (2012) 22339771

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)UNEGFPmouse Cacng5 promoter

Ordering Information

Donor DNAmouse BAC clone (RP23-329L1) containing mouse Cacng5 genomic DNA, jellyfish enhanced green fluorescent protein cDNA, bovine growth hormone polyadenylation signal, E. coli ampicilline resistance gene, E. coli R6Kgamma origin of replication
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Eur. J. Neurosci., 35, 702-710 (2012). The use of the BIOLOGICAL RESOURCE is strictly limited to academic, non-profit research institutes for academic non-profit research purposes. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, a mention of the GENSAT Project (GENSAT1-BX1300) as the source of the original BAC transgenic construct are requested.
DepositorHajime Hirase (RIKEN)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data