Strain Information | |
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Image | |
BRC No. | RBRC06297 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | CAJcl.Cg-Aicda<tm1Hon> |
Former Common name | BALBcAJcl-AID KO, BALBcA-AID KO |
H-2 Haplotype | |
ES Cell line | TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] |
Background strain | |
Appearance | |
Strain development | Developed by Reiko Shinkura, Nagahama Institute of Bio-Science and Technology, and Tasuku Honjo, Graduate School of Medicine, Kyoto University. TT2 ES cells were used. The mutant mice were backcrossed to BALB/cAnPt over 5 times, and then nackcrossed to BALB/cAJcl. |
Strain description | Aicda knockout mice. BALB/cAJcl background. Exon 2 and part of Exon 3 were replaced with a neomycin resistant cassette. For the details, please contact the developer (Dr. Reiko Shinkura, Nagahama Institute of Bio-Science and Technology). |
Colony maintenance | Homozygote x Homozygote [or Crossing to BALB/cAJcl] |
References | Cell, 102, 553-563 (2000). 11007474 Science, 298, 1424-1427 (2002). 12434060 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)6mouse phosphoglycerate kinase promoter (PGK promoter) |
Ordering Information | |
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Donor DNA | Phage P1 loxP sites, E. coli Neomycin resistance gene, Mouse Phosphoglycerate kinase promoter (PGK promoter), mouse Aicda genomic DNA |
Research application | Cre/loxP system Immunology and Inflammation Research |
Specific Term and Conditions | 1) The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results. 2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of the following literature(s) designated by the DEPOSITOR are requested. Science, 298, 1424-1427 (2002). 3) RECIPIENT shall notify the PROVIDER upon filing a patent application claiming modification of the BIOLOGICAL RESOURCE or method(s) of manufacture or use(s) of the BIOLOGICAL RESOURCE. |
Depositor | Tasuku Honjo (Kyoto University) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |