Strain Information | |
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Image | |
BRC No. | RBRC09257 |
Type | Transgene |
Species | Mus musculus |
Strain name | C.Cg-Tg(CAG-tdKaede)15Utr |
Former Common name | Kaede (WK-m15), BALB-CAG-Kaede Tg#15 |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Developed by Michio Tomura, Kyoto University Graduate School of Medicine and Yoshihiro Miwa, Graduate School of Comprehensive Human Sciences, University of Tsukuba. The transgene was injected into the pronuclei of B6D2F1 fertilized eggs. The Kaede cDNA was provided by Atsushi Miyawaki, RIKEN Brain Science Institute. The Tg mice were backcrossed to BALB/c over 22 times. |
Strain description | These transgenic mice expressing a Kaede, photoconvertible fluorescence protein under the control of the CAG promoter. The expression of green fluoresce of Kaede mice can be converted to red on irradiation with UV or violet light. |
Colony maintenance | Carrier x Noncarrier [BALB/cAJcl] |
References | Proc. Natl. Acad. Sci. USA, 105(31):10871-10876 (2008). 18663225 |
Health Report | |
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Examination Date / Room / Rack | 2024/02/26Room:4-BRack:A |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter tdKaedeTrachyphyllia geoffroy Kaede cDNAUNtdKaedeCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 polyA signalUN |
Ordering Information | |
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Donor DNA | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Trachyphyllia geoffroy Kaede cDNA, SV40 Poly(A) site |
Research application | Fluorescent Proteins/lacZ System |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc Natl Acad Sci U S A. 105, 10871-10876 (2008). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER of the Kaede using Approval form (form V) (Lab Contact: Laboratory for Cell Function Dynamics, RIKEN CBS: mta-cfds@ml.riken.jp). |
Depositor | Yoshihiro Miwa (University of Tsukuba) |
Strain Status | Live mice Frozen embryos Frozen sperm |
Strain Availability | Recovery and QC required prior to distribution Cryopreserved sperm (within 1 month) |
Additional Info. | Mouse of the Month Dec 2020 Genotyping protocol -PCR- Necessary documents for ordering: |
BRC mice in Publications |
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No Data |