Strain Data Sheet

RBRC09475

Strain Information

Image
BRC No.RBRC09475
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S2-Piwil2<tm1Nkn>
Former Common nameMili-KO
H-2 Haplotype
ES Cell lineD3 [129S2/SvPas]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
ReferencesDevelopment., 131, 839-849 (2004). 14736746

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Piwil2piwi-like RNA-mediated gene silencing 214Piwil2<tm1Nkn>targeted mutation 1, Toru Nakano

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)14mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

Donor DNAE. coli Neomycin resistance gene, mouse Phosphoglycerate kinase (Pgk1) promoter and polyA, mouse Mili (mouse PiwiL2)genomic DNA
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Development., 131, 839-849 (2004).Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR (by e-mail).
DepositorToru Nakano (Osaka University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data