Strain Information | |
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Image | |
BRC No. | RBRC09648 |
Type | Transgene |
Species | Mus musculus |
Strain name | C57BL/6-Htr5a<tm1(icre)Ahky> |
Former Common name | 5Ht5a-iCre |
H-2 Haplotype | |
ES Cell line | |
Background strain | C57BL/6JJcl |
Appearance | |
Strain development | Developed by Akihiro Yamanaka, Nagoya University. ES cells derived from C57BL/6N were used. Mice were maintained in C57BL/6 background. |
Strain description | Cre knock-in mice at the 5Ht5a locus. This line may be useful for Cre-loxP recombination in 5ht5a-positive cells. A pgk-neo cassette still remains downstream of the Cre sequence. |
Colony maintenance | |
References |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Htr5a5-hydroxytryptamine (serotonin) receptor 5A5Htr5a<tm1 (icre)Ahky>targeted mutation 1, Akihiro Yamanaka Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site5Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site5Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter WPREWoodchuck hepatitis virus posy-transcriptional regulatory element (WPRE)5WPRE Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter iCrephage P1 codon improved Cre (iCre)5iCre Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)5mouse phosphoglycerate kinase promoter (PGK promoter) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 PolyA signal5 |
Ordering Information | |
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Donor DNA | phage P1 Cre recombinase, woodchuck hepatitis virus WPRE (woodchuck hepatitis virus posttranscriptional regulatory element) sequence, SV40 polyA signal, yeast FRT (flipase recombination target) sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse pgk-PolyA, mouse Htr5a genomic DNA |
Research application | Cre/loxP system FLP/frt system |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced.) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. |
Depositor | Akihiro Yamanaka (Nagoya University) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering: |
BRC mice in Publications |
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No Data |