Strain Data Sheet

RBRC09661

Strain Information

Image
BRC No.RBRC09661
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Gt(ROSA)26Sor<tm12.1(Nphs1-EGFP)Maoh>
Former Common nameNephrin-EGFP mice
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Masato Ohtsuka and Shinji Hadano, Tokay University in 2012.
Strain descriptionKnock-in mice of a DNA construct, Nephrin-eGFP-poly A, at the Rosa26 locus. This strain was developed with the PITT method invented by depositors. The mice were originated from the E14 ES cell line (129P2) and then maintained in C57BL/6 background for more than 5 generations.
Colony maintenance
ReferencesPLoS One, 11(6):e0157497 (2016). 27362433

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm12.1(Nphs1-EGFP)Maoh>targeted mutation 12.1, Masato Ohtsuka

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)6EGFPmouse Nephrin promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site6Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SASplice acceptor6SA

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lox2272phage P1 loxP variant6lox2272

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA signal6

Ordering Information

Donor DNAadenovirus Splicing Acceptor, yeast FRT site, Simian virus 40 (SV40) polyA, jellyfish eGFP cDNA, mouse Nephrin promoter, phage P1 loxP variant (lox2272), mouse Gt(ROSA)26Sor genomic DNA
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced.) For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorMasato Ohtsuka (Tokai University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data