Strain Data Sheet

RBRC09695

Strain Information

Image
BRC No.RBRC09695
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Rnf31<tm1.2Kiwa>
Former Common nameHOIP delta linear mice
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Kazuhiro Iwai, Graduate School of Medicine, Kyoto University. TT2 ES cell line [(C57BL/6 x CBA)F1] was used. A pair of loxP sites were flanking an exon encoding RING-IBR-RING domain, and a genomic fragment between loxP sites were deleted by CAG-Cre. A Neo resistance gene was also deleted. C57BL/6 congenic strain.
Strain descriptionRnf31 (ring finger protein 31, HOIP) deficient mice. Exon encoding a RING-IBR-RING domain was deleted. Homozygous mutants are lethal at embryonic day 10.5.
Colony maintenance
ReferencesMol. Cell Biol., 36, 1569-1583 (2016). 26976635

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Rnf31ring finger protein 3114Rnf31<tm1.2Kiwa>targeted mutation 1.2, Kazuhiro Iwai

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP14loxP

Ordering Information

Donor DNAPhage P1 loxP site, mouse Rnf31 genomic DNA
Research applicationCell Biology Research
Cre/loxP system
Immunology and Inflammation Research
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Cell Biol., 36, 1569-1583 (2016).The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE
DepositorKazuhiro Iwai (Kyoto University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data