Strain Information | |
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Image | |
BRC No. | RBRC09740 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.Cg-Ddhd1<tm1b(KOMP)Katn> |
Former Common name | |
H-2 Haplotype | |
ES Cell line | TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] |
Background strain | C57BL/6JJcl |
Appearance | |
Strain development | Developed by Katsuko Tani, Tokyo University of Pharmacy and Life Sciences. TT2 (B6/CBA) ES cell line was used. C57BL/6J congenic line. |
Strain description | Ddhd1 (DDHD domain containing 1) knockout mice. RBRC09738 is a strain in which FRT-flanked lacZ/neomycyin fusion gene (geo) followed by a loxP site was inserted in front of exon 8 and another loxP site was inserted after the exon 8. RBRC09740 is a strain in which RBRC09738 was futher crossed with CAG-Cre to delete the exon 8, and then backcrossed to C57BL/6J. Homozygous male mice showed subfertility. A strain in mixed genetic background of C57BL/6 and CBA is available as RBRC09739. |
Colony maintenance | |
References | J. Biol. Chem., 289, 11497-11511 (2014). 24599962 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site14Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lacZbeta-galactosidase (E. coli)14 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP14loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)14 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 polyA14 |
Ordering Information | |
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Donor DNA | yeast FRT (flipase recombination target) sites, Mouse En2 intron 2/exon 3 splice acceptor sequence, E.coli lacZ, E.coli neo, SV40 PolyA, Bacteriophage P1 loxP site, mouse Ddhd1 genomic DNA |
Research application | Cre/loxP system FLP/frt system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Biol. Chem., 289, 11497-11511 (2014).RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. Within a period of TWO years after the first paper concering of the BIOLOGICAL RESOURCE is published, the RECIPIENT agree to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the Knockout Mouse Project and the KOMP Repository as the source of the original allele and mice are requested. |
Depositor | Mitsuo Tagaya (Tokyo University of Pharmacy and Life Sciences) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |