Strain Data Sheet

RBRC09773

Strain Information

Image
BRC No.RBRC09773
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S4-Birc6<tm1Nai>
Former Common nameApollon knockout mice
H-2 Haplotype
ES Cell lineJ1 [129S4/SvJae]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Mikihiko Naito, The University of Tokyo, and Tetsuo Noda, The Cancer Institute of JFCR in 2002. E14 ES cell lines were used. C57BL/6 congenic strain. A floxed Neomycin resistance gene was removed by crossing to CAG-Cre line in B6 genetic background.
Strain descriptionBirc6(baculoviral IAP repeat-containing 6)knockout mice. Homozygous mutants are embryonically or neonatally lethal. Heterozygous mice are born normally.
Colony maintenance
ReferencesNat. Cell Biol., 6, 849-860 (2004). 15300255
J. Biol. Chem., 289, 3457-3467 (2014). 24302728

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Birc6baculoviral IAP repeat-containing 617Birc6<tm1Nai>targeted mutation 1, Mikihiko Naito

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)17

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP17loxP

Ordering Information

Donor DNASV40 Large T antigen nuclear localization signal(NLS), E. coli Beta galactosidase gene, phage P1 loxP site, mouse Birc6 genomic DNA
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Cell Biol., 6, 849-860 (2004). J. Biol. Chem., 289, 3457-3467 (2014).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorMikihiko Naito (National Institute of Health Sciences)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data