Strain Data Sheet

RBRC09774

Strain Information

Image
BRC No.RBRC09774
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Kdm7a<tm1.1Yts>
Former Common nameKDM7A KO
H-2 Haplotype
ES Cell lineRENKA [C57BL/6NCrlCrlj]
Background strain
Appearance
Strain developmentDeveloped by Yuichi Tsukada, Kyushu University in 2014. RENKA ES cell line (C57BL/6)was used. C57BL/6 congenic strain.
Strain descriptionKdm7a [lysine (K)-specific demethylase 7A] knockout mice. Exon 6 is deleted. A targeting vector containing loxP-Exon 6-FRT-pgk-neo-FRT-loxP was used. A DNA fragment between loxP sites was deleted by crossing a Cre mouse line.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Kdm7alysine (K)-specific demethylase 7A6Kdm7a<tm1.1Yts>targeted mutation 1.1, Yuichi Tsukada

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Ordering Information

Donor DNABacteriophage P1 loxP site, mouse Kdm7a genomic DNA
Research applicationCre/loxP system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
DepositorYuichi Tsukada (Kyushu University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data