Strain Data Sheet

RBRC09795

Strain Information

Image
BRC No.RBRC09795
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameB6D2-Fmr1nb<em1Osb>
Former Common nameFmr1nb<delta 1/Y>
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Ayako Isotani and Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2014. BDF1 background.
Strain descriptionFmr1nb mutant mice generated by the CRISPR/Cas9 technique. Fmr1nb<em1Osb> [Deleted sequence]:1 bp in Exon1 CTGAGAGTTTTAAGTCCCAGGAGGTTCTGGACTGCTCGCAAGCGGTGGTGCGTCACTATCACCTCAGGTTGGCTGGTTAGAGACCTCCAACATGCCTTCGGATCGAAGGCCAAGTCAGAGAAGGAATAGATCTAAGAGCCGTGATTATCGTGGTGCACGGTCAAAGGTAACAAGAGCTGATACGAGGAACAGAGACGATACTCTTGCCCTCAGTATGTATCAGGGGCCTCCGAGTGCCGACCAGGGGAACAACATGGCGGATGCCCCTCGGTTTGGCTTCTGGACTTCAGTAAGCCAGTGTCTGCAATACTTGTGGGCCAGGAGGCACTTGGGCCTGCTTCTACTTTTATTCTGGACGCTGGTGATCCTGTTCCGTCCTGTGAACAC
Colony maintenance
ReferencesProc. Natl. Acad. Sci. USA, 113(28):7704-10 (2016). 27357688

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Fmr1nbfragile X mental retardation 1 neighborXFmr1nb<em1Osb>endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University

Ordering Information

Donor DNApX330-U6-Chimeric_BB-CBh-hSpCas9[human U6 promoter, S. pyogenes gRNA scaffold, human U6 terminator, CMV,chicken hybrid CMV enhancer/chicken beta-actin promoter (CBh), Synthetic DNA 3xFLAG, SV40 nuclear localization signal(NLS), Streptococcus pyogenes SpCas9 (human codon-optimized), bovine GH polyA signal, AAV2 inverted terminal repeat (ITR), f1 phage f1 origin, E. coli Ampicillin resistance gene (AmpR), E. coli pUC origin], Mouse a part of Fmr1nb gene
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 113(28):7704-10 (2016). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasahito Ikawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP

BRC mice in Publications

No Data