Strain Data Sheet

RBRC09798

Strain Information

Image
BRC No.RBRC09798
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Ano5<tm1.2Naga>
Former Common nameTMEM16E KO
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Shigekazu Ngata, Jun Suzuki and Sayuri Gyobu at Osaka University in 2012. Bruce 4 ES cell line was used. Exon 2 and Neomycin resistance gene were removed by crossing CAG-Cre mice.
Strain descriptionAno5(anoctamin 5, Tmem16e) knockout mice.
Colony maintenance
ReferencesMol. Cell Biol., 36, 645-659 (2015). 26667038
Neuromuscular Disorders, 25, 533-541 (2015). 25891276

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Ano5anoctamin 57Ano5<tm1.2Naga>targeted mutation 1.2, Shigekazu Nagata

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP7loxP

Ordering Information

Donor DNAPhage P1 loxP site, mouse Ano6 genome DNA
Research applicationCre/loxP system
Mouse Models for Human Disease
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Cell Biol., 36, 645-659 (2015).The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorShigekazu Nagata (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-
Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP

BRC mice in Publications

Li X, Wang L, Wang H, Qin A, Qin X.
Ano5 modulates calcium signaling during bone homeostasis in gnathodiaphyseal dysplasia.
NPJ Genom Med 7(1) 48(2022) 35982081