Strain Information | |
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Image | |
BRC No. | RBRC09859 |
Type | Transgene |
Species | Mus musculus |
Strain name | C57BL/6J-Tg(CMV-cat,-SDHA<*>Y215F)1Hom |
Former Common name | CATFL-SDHAY215F Tg |
H-2 Haplotype | |
ES Cell line | |
Background strain | C57BL/6JJcl |
Appearance | |
Strain development | Developed by Yoshimi Homma, Fukushima Medical University in 2012. C57BL/6 genetic background. |
Strain description | Transgenic mice expressing mutated SDHA (Succinate dehydrogenase A, Y215F) in a Cre-dependent manner. Floxed cat (chloramphenicol acetyltransferase) gene and SDHA(Y215F) are driven by the CMV promoter. |
Colony maintenance | |
References | Biochem. J., 447(2), 281-289 (2012). 22823520 Eur. J. Immunol., 47, 406-418 (2017). 27883180 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter FLAGFLAG tag (synthetic)UNFLAG Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SDHAsuccinate dehydrogenase complex, subunit A, flavoprotein (Fp) (human)UNSDHA Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter catE. coli chloramphenicol acetyltransferase (CAT)UN Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxPUNloxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxPUNloxPCMV promoter, rabbit beta-globin intron, rabbit beta-globin poly A Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter poly Apolyadenylic acid additional signalUNpoly A |
Ordering Information | |
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Donor DNA | Cytomegalovirus CMV promoter, rabbit beta globin intron, Phage P1 loxP sites, E. coli chloramphenicol acetyltransferase (CAT) gene, rabbit beta globin polyA, Human SDHA gene cDNA, FLAG epitope tag(synthetic), pA |
Research application | |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Eur. J. immunol., 47, 406-418 (2017). |
Depositor | Yoshimi Honma (Fukushima Medical University) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |