Strain Data Sheet

RBRC09905

Strain Information

Image
BRC No.RBRC09905
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameB6D2-Anxa10<em1Siag>
Former Common nameAnxa10 -/-
H-2 Haplotype
ES Cell line
Background strainJcl:B6D2F1
Appearance
Strain developmentDeveloped by Shinji Sasaki, Shirakawa Institute of Animal Genetics, Japan Livestock Technology Association in 2016. This strain was generated by the CRISPR/CAS9 technique. B6D2 background.
Strain descriptionAnxa10 (annexin A10) mutant mice. 8 bp deletion at exon 2. Anxa10<em1Siag>: 8 bp deletion at exon 2.tttcatggaaattcatacaatctcttaagtgttgatttttttttcccgacagGTCCAAGGAACCATCTTCCCAGCTCCAAATTTCAATCCCATGATGGATGCCCAAATGCTAGGCGGAGCACTCCAAGGATTTGgtaagccttggaaatacattcgttgattatgaaaaaatataacgtaaaagtaaatttctaggcctttaggcccaggcttgagaatgtgacctctatgactcaatgctccaaggt
Colony maintenance
ReferencesBMC Genomics, 17, 968- (2016). 27881083

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Anxa10annexin A108Anxa10<em1Siag>endonuclease-mediated mutation 1, Shinji Sasaki

Ordering Information

Donor DNApX330-U6-Chimeric_BB-CBh-hSpCas9[human U6 promoter, S. pyogenes gRNA scaffold, human U6 terminator, CMV,chicken hybrid CMV enhancer/chicken beta-actin promoter (CBh), Synthetic DNA 3xFLAG, SV40 nuclear localization signal(NLS), Streptococcus pyogenes SpCas9 (human codon-optimized), bovine betaGH polyA signal, AAV2 inverted terminal repeat (ITR), f1 phage f1 origin, E. coli Ampicillin resistance gene (AmpR), E. coli pUC origin]
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. BMC Genomics, 17, 968- (2016).
DepositorYoshikazu Sugimoto (Japan Livestock Technology Association)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data