Strain Data Sheet
RBRC10163
Strain Information | |
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| Image | |
| BRC No. | RBRC10163 |
| Type | Transgene and Spontaneous Mutation |
| Species | Mus musculus |
| Strain name | HR1.B6-Hr<hr> Tg(EF1A-PDHL/roGFP1)20Awolf |
| Former Common name | Mito-roGFP mice(MROH20) |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | Developed by Alexander M Wolf, G Nippon Medical School in 2012. Transgenic construct was injected into fertilized eggs derived from C57BL/6J. Mice were backcrossed to Hos:HR-1 for more than 10 generations. Mice were maintained as heterozygote. |
| Strain description | Transgenic mice expressing ratiometric redox-sensitive GFP (PDHL/roGFP1) in the mitochondria of several tissue under the human EF1A promoter. Redox states can be monitored at skin. |
| Colony maintenance | |
| References | J. Invest. Dermatol., 134(6), 1701-1709 (2014). 24129062 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter CSF3human G-CSF cDNA PolyA signalUNCSF3 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter PDHA1mitchondrial targeting seaquence (human pyruvate dehydrogenase E1 subunit leader sequence)UNPDHA1human elongation factor 1 alpha (EF-1 alpha) promoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter roGFPRedox-sensitive Green Fluorescent Protein (Aequorea victoria)UNroGFP |
Ordering Information | |
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| Donor DNA | human EF1A promoter, mitchondrial targeting seaquence (human pyruvate dehydrogenase E1 subunit leader sequence, PDHA1), jellyfish GFP(EGFP) cDNA, human G-CSF cDNA PolyA signal |
| Research application | Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Invest. Dermatol.,134(6), 1701-1709 (2014).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
| Depositor | Alexander M. Wolf (Nippon Medical School) |
| Strain Status |  Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |