Strain Data Sheet
ANIMAL SEARCH SYSTEM
Data update: Jun 20, 2018
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RBRC No. RBRC10189  
Type Targeted MutationCartagena  
Species Mus musculus  
Strain name C57BL/6-Gt(ROSA)26Sor<tm1.1(CAG-3XFLAG/dCas9,-EGFP)Hfuj>  
Former Common name 3×FLAG-dCas9/CTV: GFP (+)  
H-2 Haplotype No Data  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain description No Data  
Health Report No Data  
Gene Details
Promoter No Data  
 1 Symbol Gt(ROSA)26Sor  
Symbol name gene trap ROSA 26, Philippe Soriano  
Chromosome 6  
Common name No Data  
Symbol description No Data  
 2 Symbol No Data  
Symbol name SV40 polyA  
Chromosome 6  
Common name No Data  
Symbol description No Data  
 3 Symbol EGFP  
Symbol name Enhanced Green Fluorescent Protein, F64L and S65T (Jellyfish)  
Chromosome 6  
Common name No Data  
Symbol description No Data  
 4 Symbol Ires  
Symbol name internal ribosomal entry site (EMCV)  
Chromosome 6  
Common name No Data  
Symbol description No Data  
 5 Symbol dCas9  
Symbol name Streptococcus pyogenes Cas9 mutant  
Chromosome 6  
Common name No Data  
Symbol description No Data  
 6 Symbol frt  
Symbol name yeast FRT (flippase recombination target) site  
Chromosome 6  
Common name No Data  
Symbol description No Data  
 7 Symbol loxP  
Symbol name phage P1 loxP  
Chromosome 6  
Common name No Data  
Symbol description No Data  
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)  
 8 Symbol No Data  
Symbol name 3xFLAG tag  
Chromosome 6  
Common name No Data  
Symbol description No Data  
References No Data  
Research applications Cre/loxP system,
FLP/frt system,
Fluorescent Proteins/lacZ System  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Transgenic mouse lines expressing the 3xFLAG-dCas9 protein for enChIP analysis. Fujita T, Kitaura F, Oji A, Tanigawa N, Yuno M, Ikawa M, Taniuchi I, and Fujii H. bioRxiv (2017) doi: https://doi.org/10.1101/221820
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.  
Additional information
 1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.  
Depositor Fujii, Hodaka (Hirosaki University)  Fujii, Hodaka 
Strain Status /
Availability
(Expected delivery)

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