|
RBRC No. |
RBRC10189
|
|
Type |
Targeted Mutation |
 |
|
Species |
Mus musculus |
|
Strain name |
C57BL/6-Gt(ROSA)26Sor<tm1.1(CAG-3XFLAG/dCas9,-EGFP)Hfuj> |
|
Former Common name |
3×FLAG-dCas9/CTV: GFP (+) |
|
H-2 Haplotype |
No Data |
|
Background strain |
No Data |
|
Appearance |
| 1 |
Appearance |
No Data |
| Genotype |
No Data |
|
Strain description |
No Data |
|
Health Report |
No Data |
|
Gene Details |
|
Promoter |
No Data |
| 1 |
Symbol |
Gt(ROSA)26Sor |
| Symbol name |
gene trap ROSA 26, Philippe Soriano |
| Chromosome |
6 |
| Common name |
No Data |
| Symbol description |
No Data |
| 2 |
Symbol |
No Data |
| Symbol name |
SV40 polyA |
| Chromosome |
6 |
| Common name |
No Data |
| Symbol description |
No Data |
| 3 |
Symbol |
EGFP |
| Symbol name |
Enhanced Green Fluorescent Protein, F64L and S65T (Jellyfish) |
| Chromosome |
6 |
| Common name |
No Data |
| Symbol description |
No Data |
| 4 |
Symbol |
Frt |
| Symbol name |
yeast FRT (flippase recombination target) site |
| Chromosome |
6 |
| Common name |
No Data |
| Symbol description |
No Data |
| 5 |
Symbol |
Ires |
| Symbol name |
internal ribosomal entry site (EMCV) |
| Chromosome |
6 |
| Common name |
No Data |
| Symbol description |
No Data |
| 6 |
Symbol |
dCas9 |
| Symbol name |
Streptococcus pyogenes Cas9 mutant |
| Chromosome |
6 |
| Common name |
No Data |
| Symbol description |
No Data |
| 7 |
Symbol |
loxP |
| Symbol name |
phage P1 loxP |
| Chromosome |
6 |
| Common name |
No Data |
| Symbol description |
No Data |
|
Promoter |
CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) |
| 8 |
Symbol |
No Data |
| Symbol name |
3xFLAG tag |
| Chromosome |
6 |
| Common name |
No Data |
| Symbol description |
No Data |
|
References |
No Data |
|
Research applications |
Cre/loxP system,
FLP/frt system,
Fluorescent Proteins/lacZ System |
|
Specific Term and Conditions |
The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Transgenic mouse lines expressing the 3xFLAG-dCas9 protein for enChIP analysis. Fujita T, Kitaura F, Oji A, Tanigawa N, Yuno M, Ikawa M, Taniuchi I, and Fujii H. bioRxiv (2017) doi: https://doi.org/10.1101/221820
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
|
Additional information |
|
1 |
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B. |
|
Depositor |
Fujii, Hodaka (Hirosaki University)  |
|
Strain Status /
Availability
(Expected delivery) |
|
